Sf9 Insect Cell Serum-Free Powder Medium

CCM-SC-0002 is a dry-format, serum-free insect cell culture medium designed for users who need a defined supply item for Sf9 suspension or adherent workflows and BTI-TN-5B1-4 insect cell platforms. The formulation is animal-origin-free and animal-derived component-free, supporting procurement requirements where undefined serum components are excluded from upstream development. It is supplied as powder for preparation of 50 L of complete medium according to the user’s validated water quality, mixing, filtration, and in-process control procedures.

The medium includes D-glucose and 1.5 mM L-glutamine and is manufactured without phenol red, which can be useful when optical readouts, color-sensitive assays, or process monitoring methods require reduced background from indicator dyes. Recommended culture conditions are 27–28 °C in an air-regulated, non-CO2 atmosphere. Endotoxin specification is ≤3 EU/mL, and the stated shelf life is 24 months when stored as directed. For adjacent defined media options, review our specialized cell culture media catalog.

• Maintenance and expansion of Sf9 insect cells in serum-free culture systems.
• Culture of BTI-TN-5B1-4 insect cell platforms used in baculovirus expression and recombinant protein development.
• Animal-origin-free media sourcing for upstream process development, scale-up studies, and comparative media evaluation.
• Phenol red-free culture conditions for workflows that use absorbance, fluorescence, imaging, or color-sensitive process analytics.
• Powder media inventory planning where long shelf life, reduced liquid storage burden, and controlled in-house reconstitution are preferred.

Store unopened powder at 2–8 °C protected from light. The product ships on blue ice to support temperature-controlled transit, and receiving teams should inspect the shipment promptly, record condition on arrival, and transfer material to qualified refrigerated storage. Use appropriate PPE and a controlled weighing and mixing environment to minimize moisture uptake and particulate exposure during preparation. After reconstitution, apply your site’s validated sterile filtration, hold-time, pH, osmolality, and microbial monitoring procedures before use in cell culture operations.

CellCultureMedia supplies CCM-SC-0002 for research and bioprocessing use with specification-focused documentation for procurement and technical review. Manufacturing and release controls are ISO-aligned, with lot-level checks appropriate for defined cell culture media, including endotoxin against the ≤3 EU/mL specification. A Certificate of Analysis is available on request through our quality documentation channel, and additional lot details such as release date, shelf life, and applicable test results can be reviewed during sourcing. For volume requirements, delivery planning, or qualification quantities, submit a request for quote; free worldwide shipping can be included in the commercial offer where applicable.