Serum-Free Hematopoietic Stem Cell Expansion Medium

This serum-free hematopoietic stem cell medium is intended for controlled culture workflows where lot consistency, defined performance specifications, and documentation are important to procurement and process teams. The product is supplied in liquid form and does not require serum addition, reducing variability associated with serum supplementation while supporting standardized experimental design. The formulation contains D-glucose as a carbohydrate source, alanyl-glutamine as a stable glutamine source, and HEPES for buffering support during handling outside the incubator. It is phenol red-free, which can be useful when colorimetric interference is undesirable in analytical or imaging workflows.

Key release specifications include pH 7.0–7.4 and osmolality 280–330 mOsmol/kg. The medium is sterile filtered, with an endotoxin specification of ≤1 EU/mL and a stated shelf life of 12 months when stored as directed. It contains animal-origin components and should be evaluated against each laboratory’s raw material policy before use in regulated process development. For related formats and complementary formulations, review our stem cell media category or contact CellCultureMedia with your target culture system, scale, and documentation requirements.

• Hematopoietic stem cell culture, expansion studies, and media comparison programs in R&D laboratories.
• Process development work requiring a serum-free, liquid medium with defined pH, osmolality, sterility, and endotoxin specifications.
• Cell and gene workflow development where phenol red-free medium is preferred for assay compatibility or optical readouts.
• Small-scale culture optimization, feeder-independent protocol assessment, and controlled CO2 incubation at 37°C with 4–6% CO2.
• Procurement standardization across academic, biotech, and biopharma teams that require a documented 500 mL ready-to-use medium format.

Store CCM-SC-0026 at 2–8°C protected from light. The product ships on blue ice to help maintain refrigerated conditions during transit. Do not freeze unless your internal validation supports freeze-thaw handling for this material. Before use, inspect the bottle for container integrity, clarity, and evidence of contamination. Warm only the volume required for the current operation, use aseptic technique, and minimize repeated temperature cycling. The assigned shelf life is 12 months when the unopened product is stored under the specified refrigerated, light-protected conditions.

CellCultureMedia supplies CCM-SC-0026 for research and bioprocessing customers who require specification-driven ordering, batch traceability, and responsive documentation support. Manufacturing and release controls are ISO-aligned, and a Certificate of Analysis is available on request through our quality documentation team. Standard purchasing is available by unit size, with support for recurring supply, consolidated shipments, and volume pricing. To confirm availability, delivery timing, or a project-specific quotation, use the request quote form. Free worldwide shipping is available for this item, subject to import requirements and destination-specific logistics review.