Serum-Free CHO Feed Supplement A

CCM-SF-0035 is a concentrated liquid feed supplement for serum-free CHO culture systems requiring animal-origin-free and animal-derived-component-free inputs. The formulation is supplied without phenol red to reduce colorimetric interference in analytical sampling and without L-glutamine to allow process engineers to control glutamine or alternative nitrogen-source strategies independently. D-glucose is included as part of the feed chemistry. This product is intended for research, process development, and bioprocessing environments using fed-batch or intensified CHO culture platforms.

Key release specifications include sterile-filtered liquid form, pH 7.0-7.4, osmolality 950-1150 mOsmol/kg, endotoxin not more than 20 EU/mL, and a stated shelf life of 12 months when stored as specified. Recommended culture conditions are 37 °C with 5-10% CO2, aligned with common suspension CHO workflows. For related options, procurement teams can review the serum-free media category or request lot and packaging details before order placement.

• Fed-batch nutrient supplementation for CHO K1, CHO-S, CHO-GS, and CHO DG44 production cultures.
• Animal-origin-free process development where defined raw material inputs and reduced adventitious-material risk are procurement priorities.
• Serum-free biopharmaceutical expression workflows requiring a phenol-red-free feed for clearer assay readouts or lower background in spent-media analysis.
• Process scale-up studies in which feed osmolality, pH contribution, and glucose delivery must be evaluated as controlled variables.
• R&D screening of feed timing, feed volume, and nutrient addition strategies in shake flasks, bench bioreactors, or scale-down models.

Store CCM-SF-0035 at 2-8 °C protected from light. Do not use the material beyond its assigned expiry date, and avoid repeated warming cycles when possible. The product ships on blue ice; upon receipt, inspect the bottle and temperature-controlled packaging, then transfer immediately to refrigerated storage. Mix gently before use if the process SOP permits, using aseptic technique throughout handling. Because the formulation is supplied without L-glutamine, any required glutamine addition should be performed according to the validated culture protocol rather than assumed as part of the feed composition.

CellCultureMedia supplies CCM-SF-0035 for B2B cell culture purchasing programs that require traceable documentation, reproducible specifications, and responsive quotation support. Manufacturing and release controls are ISO-aligned, and a Certificate of Analysis can be requested for quality review, incoming inspection, and vendor qualification files. For documentation expectations, visit quality and compliance support. For multi-bottle orders, standing replenishment, or scale-up purchasing, submit requirements through request a quote. Free worldwide shipping is available on qualified orders, with temperature-managed dispatch on blue ice.