Serum-Free ADCF Exosome Production Medium

CCM-SC-0032 is a ready-to-use serum-free, animal-derived component-free medium developed for exosome expression and extracellular vesicle collection workflows where defined composition and low background are important. The formulation is supplied as a clear liquid without phenol red, reducing colorimetric interference in downstream analytical methods and supporting culture environments where indicator dye is not desired.

The medium includes D-glucose as an energy source, L-glutamine as a nitrogen source for proliferating and metabolically active cultures, and sodium bicarbonate for CO2-buffered culture. Release specifications include pH 7.0–7.4, osmolality 280–320 mOsmol/kg, and endotoxin not more than 1 EU/mL. It is classified as animal origin-free and is intended for research, development, and bioprocessing workflows requiring controlled raw materials and lot-level documentation.

• Exosome and extracellular vesicle production studies using adherent or suspension cell culture systems that require serum-free conditions.
• Conditioned-medium collection where absence of phenol red helps reduce assay background during particle analysis, protein quantification, RNA workflows, or optical readouts.
• Stem cell, progenitor cell, and engineered cell culture processes that require animal origin-free medium inputs within defined process development programs.
• Small-scale screening, protocol transfer, and scale-up evaluation in incubators operated at 36–38 °C and 5–10% CO2.
• Procurement standardization for laboratories seeking a 500 mL liquid medium with stated pH, osmolality, endotoxin, shelf-life, and shipping conditions.

Store CCM-SC-0032 at 2–8 °C protected from light. Do not expose the bottle to repeated warming cycles; warm only the required volume or equilibrate the container under controlled laboratory procedures before use. The stated shelf life is 12 months when unopened and stored as specified. This product ships on blue ice to maintain refrigerated conditions during transit. Inspect the container on receipt, record the lot number for traceability, and follow internal aseptic handling procedures when dispensing into sterile vessels.

CellCultureMedia supplies CCM-SC-0032 for B2B research and bioprocess customers that require specification-driven purchasing. Manufacturing and release controls are ISO-aligned, with lot records supporting sterility, endotoxin, pH, osmolality, appearance, labeling, and shelf-life review. A Certificate of Analysis is available on request, and the quality documentation team can support vendor qualification and incoming material review.

The standard pack size is 500 mL, with list pricing from USD 805.56 per bottle. For recurring use, multi-bottle demand, or project-based supply planning, submit a request for quote. Free worldwide shipping is available, subject to destination requirements and applicable import documentation.