RPMI 1640 Medium with Phenol Red, Glutamine and HEPES
CCM-CM-0013 is an RPMI 1640 medium with phenol red, glutamine and HEPES in a 500 mL pack, supplied as a liquid, for distributors, resellers, marketplaces and bulk buyers. Classification as listed: animal-origin-free. Listed shelf life is 12 months, storage is 2-8 °C, protected from light, and the shipping condition is blue ice. Supply is by written quotation only.
- ✓ Wholesale and bulk pricing on request
- ✓ Private-label and OEM packaging quoted on request
- ✓ ISO-aligned manufacturing
- ✓ Certificate of Analysis on request
Wholesale supply terms
- Pack size
- Listed pack: 500 mL. Other pack sizes on request.
- Minimum order
- Minimum order quantities on request
- Bulk format
- Bulk packs quoted on request; ask whether a powder format is available
- Private label / OEM
- Private-label and OEM packaging quoted on request
- Lot reservation
- Lot reservation for repeat supply on request
- Lead time
- Quoted per order
- Documents
- Certificate of Analysis on request
We supply companies that resell to laboratories: distributors, resellers, catalogue companies, marketplaces and private-label buyers. We do not sell single units to individual labs.
Supply facts for resellers
- SKU: CCM-CM-0013
- Pack size: 500 mL
- Form: Liquid
- Classification: Animal-origin-free
- Shelf life: 12 months
- Storage: 2-8 °C, protected from light
- Shipping condition: Blue ice
Certificate of Analysis on request.
Product specification
RPMI 1640 with phenol red, glutamine and HEPES, a standard medium for suspension cells such as leukaemia lines and PBMC, with added buffering from HEPES.
- Sterility: Filter-sterilized
- Cell line (as listed): Leukemia cells, PBMC, etc.
- Serum level (as listed): Requires 10% fetal bovine serum
- Culture conditions (as listed): 37 °C, 5-10% CO2
- Endotoxin: ≤1 EU/mL
- Osmolality: 260–305 mOsmol/kg
- pH: 7.0–7.4
- Application area: Scientific and Basic Research
Related lines are listed under classical media.
What to send for a quotation
- Product and SKU (CCM-CM-0013)
- Pack size (500 mL)
- Quantity per quarter
- Repeat cadence
- Destination country
Pricing, minimums and lead time are stated in the written quotation. Private label and lot reservation can be requested; both are quoted per project or order. See the distributor programme for how we work with resellers.
Specifications
Formulation highlights
Contains (+)
Phenol red, 2.0 g/L D-glucose, 300 mg/L L-glutamine, HEPES
Storage and handling
Storage: 2~8 ℃, protected from light. Shelf life: 12 months. Shipped cold-chain where required.
Quality and documentation
Manufactured under ISO-aligned quality management systems. A Certificate of Analysis is available on request: email info@cellculturemedia.bio with the SKU.
About this product
Can I resell CCM-CM-0013 under my own label?
Can the same lot be held for repeat orders?
How is this liquid shipped and stored?
Learn more about this
- RPMI 1640 MediumRPMI 1640 is a basal cell culture medium developed at Roswell Park Memorial Institute in 1966 for the culture of human leukocytes in suspension. It contains 2,000 mg/L glucose (11.1 mM), 2,000 mg/L sodium bicarbonate (23.8 mM) buffered for a 5% CO2 atmosphere, unusually high phosphate (about 5.6 mM), low calcium (about 0.42 mM), and a distinctive component set that includes reduced glutathione, biotin, vitamin B12, para-aminobenzoic acid and hydroxyproline. It is the standard medium for lymphocytes, hybridomas, and most suspension-adapted haematopoietic and lymphoid cell lines, normally supplemented with 10% fetal bovine serum.
- HEPES buffer in cell cultureHEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic organic buffer used in cell culture to hold medium at physiological pH without depending on incubator CO2. Its pKa is about 7.5 at 20-25 degrees C and about 7.3 at 37 degrees C, giving useful buffering across roughly pH 6.8-8.2, and it is normally added to medium at 10-25 mM from a sterile 1 M stock. Because HEPES buffering does not rely on the carbonic acid/bicarbonate equilibrium, HEPES-supplemented medium resists the fast alkaline drift that occurs when a flask leaves a 5% CO2 incubator.
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