RPMI 1640 Medium with Phenol Red, Glutamine and HEPES

CCM-CM-0013 is an animal-origin-free RPMI 1640 classical medium supplied in liquid form for cell culture research and bioprocess development. The formulation contains phenol red for visual pH indication, 2.0 g/L D-glucose as a carbohydrate energy source, 300 mg/L L-glutamine for nitrogen and carbon metabolism, and HEPES for added buffering capacity during routine manipulation outside the incubator. This configuration is commonly selected when users need a ready-to-use RPMI platform with defined pH support and compatibility with serum supplementation.

The medium is specified for use with 10% fetal bovine serum or an equivalent validated supplement, depending on the cell model and internal protocol. Key release specifications include pH 7.0–7.4, osmolality 260–305 mOsmol/kg, endotoxin not more than 1 EU/mL, and filter-sterilized manufacture. The product is intended for 37 °C culture under 5–10% CO2. For adjacent formulations and procurement comparisons, see the classical media category or use request a quote for volume planning.

• Maintenance and expansion of PBMC cultures in basic research, immunology workflows, assay development, and analytical method support.
• Culture of hematopoietic suspension cell models that have been internally qualified for RPMI 1640 chemistry and serum-containing conditions.
• R&D screening where phenol red is useful as a rapid visual indicator of acidification, medium exhaustion, or handling variation.
• Routine laboratory workflows requiring a HEPES-buffered RPMI format for improved short-term pH stability during sampling, feeding, centrifugation, and plate setup.
• Process-development studies where defined incoming material specifications for pH, osmolality, sterility, endotoxin, shelf life, and shipping conditions are required for purchasing records.

Store CCM-CM-0013 at 2–8 °C protected from light. Do not freeze unless an internal validation study supports that handling condition, as freezing can affect solubility, bottle integrity, and performance consistency. The stated shelf life is 12 months when stored unopened under recommended conditions. Shipments are packed with blue ice to support temperature-controlled transit. Before use, inspect the container for leakage, turbidity, color shift beyond normal phenol red variation, or visible particulates. Warm only the required volume, use aseptic technique, and avoid repeated extended warming of the full bottle. Supplement with 10% fetal bovine serum or another qualified supplement immediately before use when required by the laboratory protocol.

CellCultureMedia supplies CCM-CM-0013 for research and bioprocessing organizations that require specification-driven purchasing and lot traceability. Manufacturing and release controls are ISO-aligned, and the medium is filter sterilized before release. Available specification data include liquid form, animal-origin-free classification, pH 7.0–7.4, osmolality 260–305 mOsmol/kg, endotoxin ≤1 EU/mL, 12-month shelf life, 2–8 °C light-protected storage, and blue-ice shipping. A Certificate of Analysis can be requested for lot-specific documentation, and additional quality information is available through CellCultureMedia quality resources. Free worldwide shipping is offered where available, with bulk pricing and scheduled supply options for multi-site labs, core facilities, and development groups.