RPMI 1640 Medium with L-Glutamine

CCM-CM-0014 is a ready-to-use liquid RPMI 1640 formulation designed for laboratories that require L-glutamine in the medium while excluding phenol red and HEPES. The formulation supports common RPMI-based culture systems, including hematopoietic suspension cell lines and peripheral blood mononuclear cell workflows, when supplemented as required by the user protocol. The source specification indicates addition of 10% fetal bovine serum for routine culture support.

This medium is supplied as an animal-origin-free liquid formulation and is sterile-filtered. Key release specifications include pH 7.0-7.4, osmolality 260-305 mOsmol/kg, endotoxin not more than 1 EU/mL, and a 12-month shelf life when stored as directed. The absence of phenol red is useful for optical, fluorescence, and color-sensitive assays where background signal or indicator color can interfere with measurement. The absence of HEPES supports protocols that rely on incubator CO2 control rather than supplemental zwitterionic buffering. For related products, see our classical media category.

• Routine culture of RPMI-adapted suspension cells under 37 degrees C and 5-10% CO2 conditions.
• PBMC handling, expansion, and assay setup in research laboratories using serum-supplemented RPMI systems.
• Color-sensitive experiments requiring a phenol red-free background for absorbance, imaging, or fluorescence readouts.
• Protocols where HEPES exclusion is preferred to maintain a defined CO2-dependent buffering environment.
• Process development, media comparison, and formulation screening where a defined RPMI 1640 base with 2.0 g/L D-glucose and 300 mg/L L-glutamine is required.

Store CCM-CM-0014 at 2-8 degrees C protected from light. Do not freeze unless the receiving laboratory has validated freeze-thaw performance for its own workflow. The product is shipped on blue ice to help maintain refrigerated conditions during transit. Before use, inspect the bottle for integrity, clarity, and absence of visible contamination. Warm only the required volume using standard aseptic technique, and minimize repeated warming of the full container. The stated shelf life is 12 months from manufacture when unopened and stored under the specified conditions.

CellCultureMedia supplies this RPMI 1640 medium through ISO-aligned manufacturing and quality workflows appropriate for research and bioprocessing supply chains. Lot-specific documentation, including a Certificate of Analysis, can be requested through our quality documentation page or during quotation. Current pack size is 500 mL, with listed price of USD 97.22 per bottle. For volume requirements, standing orders, or multi-site purchasing, contact us through request a quote. Free worldwide shipping is available for qualified orders.