RPMI 1640 Medium with L-Glutamine
RPMI 1640 Medium with L-glutamine, without phenol red or HEPES, is a sterile liquid classical cell culture medium for suspension and immune-cell workflows. Supplied as CCM-CM-0014 in a 500 mL bottle, it contains 2.0 g/L D-glucose and 300 mg/L L-glutamine and is intended for research and bioprocessing use. CellCultureMedia provides procurement support, documentation on request, and free worldwide shipping for qualified orders.
- ✓ Free worldwide shipping
- ✓ ISO-aligned manufacturing
- ✓ Certificate of Analysis on request
- ✓ Bulk & custom volumes quoted on request
Overview
CCM-CM-0014 is a ready-to-use liquid RPMI 1640 formulation designed for laboratories that require L-glutamine in the medium while excluding phenol red and HEPES. The formulation supports common RPMI-based culture systems, including hematopoietic suspension cell lines and peripheral blood mononuclear cell workflows, when supplemented as required by the user protocol. The source specification indicates addition of 10% fetal bovine serum for routine culture support.
This medium is supplied as an animal-origin-free liquid formulation and is sterile-filtered. Key release specifications include pH 7.0-7.4, osmolality 260-305 mOsmol/kg, endotoxin not more than 1 EU/mL, and a 12-month shelf life when stored as directed. The absence of phenol red is useful for optical, fluorescence, and color-sensitive assays where background signal or indicator color can interfere with measurement. The absence of HEPES supports protocols that rely on incubator CO2 control rather than supplemental zwitterionic buffering. For related products, see our classical media category.
Applications
- Routine culture of RPMI-adapted suspension cells under 37 degrees C and 5-10% CO2 conditions.
- PBMC handling, expansion, and assay setup in research laboratories using serum-supplemented RPMI systems.
- Color-sensitive experiments requiring a phenol red-free background for absorbance, imaging, or fluorescence readouts.
- Protocols where HEPES exclusion is preferred to maintain a defined CO2-dependent buffering environment.
- Process development, media comparison, and formulation screening where a defined RPMI 1640 base with 2.0 g/L D-glucose and 300 mg/L L-glutamine is required.
Storage and Handling
Store CCM-CM-0014 at 2-8 degrees C protected from light. Do not freeze unless the receiving laboratory has validated freeze-thaw performance for its own workflow. The product is shipped on blue ice to help maintain refrigerated conditions during transit. Before use, inspect the bottle for integrity, clarity, and absence of visible contamination. Warm only the required volume using standard aseptic technique, and minimize repeated warming of the full container. The stated shelf life is 12 months from manufacture when unopened and stored under the specified conditions.
Quality and Documentation
CellCultureMedia supplies this RPMI 1640 medium through ISO-aligned manufacturing and quality workflows appropriate for research and bioprocessing supply chains. Lot-specific documentation, including a Certificate of Analysis, can be requested through our quality documentation page or during quotation. Current pack size is 500 mL, with listed price of USD 97.22 per bottle. For volume requirements, standing orders, or multi-site purchasing, contact us through request a quote. Free worldwide shipping is available for qualified orders.
Specifications
Formulation Highlights
Contains (+)
2.0 g/L D-glucose, 300 mg/L L-glutamine
Does Not Contain (-)
Phenol red, HEPES
Storage & Handling
Storage: 2~8 ℃, protected from light. Shelf life: 12 months. Ship cold-chain where required.
Quality & Documentation
Manufactured under ISO-aligned quality management systems. Full documentation — Certificate of Analysis (CoA), Material Safety Data Sheet (MSDS), formulation summary, and stability data — is available on request. Email [email protected] with the SKU.
About this product
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Learn more about this
- RPMI 1640 MediumRPMI 1640 is a basal cell culture medium developed at Roswell Park Memorial Institute in 1966 for the culture of human leukocytes in suspension. It contains 2,000 mg/L glucose (11.1 mM), 2,000 mg/L sodium bicarbonate (23.8 mM) buffered for a 5% CO2 atmosphere, unusually high phosphate (about 5.6 mM), low calcium (about 0.42 mM), and a distinctive component set that includes reduced glutathione, biotin, vitamin B12, para-aminobenzoic acid and hydroxyproline. It is the standard medium for lymphocytes, hybridomas, and most suspension-adapted haematopoietic and lymphoid cell lines, normally supplemented with 10% fetal bovine serum.
- Phenol Red in Cell Culture MediaPhenol red (phenolsulfonphthalein) is a pH indicator added to cell culture media at roughly 5-15 mg/L, where it turns yellow below about pH 6.8 and pink to fuchsia above about pH 8.2, with the normal red-orange of healthy medium sitting near pH 7.4. Medium turning yellow means acidification - almost always lactate from cell metabolism, from an overgrown culture or from bacterial contamination - while medium turning pink or purple means the medium has gone alkaline, nearly always because CO2 has escaped from the bicarbonate buffer. Phenol red does no buffering itself and is purely diagnostic; use phenol-red-free medium for fluorescence and absorbance assays, and for oestrogen-responsive cell work, where the dye interferes with the readout.
