Reduced-Serum Transfection Medium

Reduced-serum transfection medium is designed to support nucleic acid delivery workflows in a broad range of mammalian cells while maintaining practical handling conditions for R&D laboratories and process development teams. CCM-SF-0066 is a sterile-filtered liquid medium formulated for use with multiple suspension and adherent mammalian cell types. The medium is phenol-red containing, which enables visual monitoring of pH shift during setup and incubation. It includes 2.5 g/L D-glucose as an energy source, 4 mM L-glutamine for nitrogen metabolism, 0.11 g/L sodium pyruvate, and HEPES to strengthen buffering during manipulations outside the incubator.

The product is classified as animal-origin-free in its supplied formulation; however, the intended working protocol requires supplementation with 5% fetal bovine serum. Recommended culture conditions are 37°C in a 5–10% CO2 incubator. Specification ranges are pH 7.0–7.4 and osmolality 280–320 mOsmol/kg, supporting compatibility with common mammalian cell culture environments. Endotoxin is controlled to ≤1 EU/mL, and the product is supplied sterile by filtration. For related formulations, review the serum-free and reduced-serum media category or request formulation support for a defined workflow.

• Reduced-serum transfection setup for adherent mammalian cells requiring a balanced salt, glucose, glutamine, pyruvate, and HEPES-containing environment.
• Suspension mammalian cell culture workflows where transient reagent exposure, incubation stability, and consistent pH control are important to experimental repeatability.
• Research-scale plasmid DNA, RNA, or reagent-complex delivery workflows where cells are maintained in a lower-serum medium supplemented with 5% fetal bovine serum.
• Process development screening where procurement teams need a defined 500 mL liquid format, controlled endotoxin specification, sterile filtration, and documented shelf life.
• Academic, biotech, and biopharma laboratory use in scientific and fundamental research settings, including optimization of cell density, incubation time, and transfection reagent ratio.

Store CCM-SF-0066 at 2–8°C protected from light. Do not use the medium beyond its 12-month shelf life, and avoid repeated warming cycles that can affect pH, amino acid stability, and overall performance consistency. Product is shipped on blue ice; upon receipt, confirm that the bottle remains cold, inspect the container for leakage or turbidity, and transfer it promptly to refrigerated storage. Before use, mix gently by inversion and warm only the required volume to room temperature or 37°C according to the validated laboratory protocol. Aseptic technique is required after opening because the medium is sterile-filtered and intended for cell culture use.

CellCultureMedia supplies CCM-SF-0066 for research and bioprocessing customers that require specification-driven purchasing and lot traceability. Manufacturing and release controls are aligned with ISO-style quality management practices, including sterile filtration, pH verification, osmolality range control, and endotoxin testing to ≤1 EU/mL. A Certificate of Analysis is available on request through our quality documentation team, and procurement teams can use the request a quote page for volume pricing, shipping confirmation, and standing-order planning. Each 500 mL unit is priced at $219.44, with free worldwide shipping available from CellCultureMedia and cold-chain shipment on blue ice.