Phenol Red-Free MSC Basal Medium II

Phenol Red-Free MSC Basal Medium II is a liquid basal medium intended for mesenchymal stem cell culture workflows that require an animal-derived component-free formulation and no phenol red indicator. The absence of phenol red is useful for laboratories that monitor optical, fluorescence, colorimetric, or endocrine-sensitive assay readouts and want to reduce interference from pH indicator dye. The animal origin-free classification supports risk-managed procurement for R&D groups, translational research organizations, and bioprocess development teams that standardize raw material inputs across multiple lots.

CCM-SC-0018 is supplied in a 500 mL bottle and specified with pH 7.0–7.4, osmolality 280–320 mOsmol/kg, and endotoxin not more than 3 EU/mL. Recommended culture conditions are 36–38 °C in a 4–6% CO2 incubator, subject to the validated protocol for the cell source, seeding density, supplements, and vessel format. This product is positioned within CellCultureMedia’s stem cell media portfolio for buyers seeking defined, procurement-ready media inputs for MSC expansion, assay preparation, and media-comparison studies.

• Basal medium component for mesenchymal stem cell culture protocols using separately qualified supplements, growth factors, attachment substrates, or serum-free additives.
• Phenol red-free culture environments for optical density, fluorescence, imaging, metabolic, and secreted-factor assays where background color may affect measurement consistency.
• Animal origin-free raw material strategies for process development teams reducing animal-derived inputs in research-scale and pilot-scale workflows.
• Lot-controlled media evaluations comparing MSC morphology, attachment behavior, expansion kinetics, and post-culture analytical readouts under defined incubation conditions.
• Academic, biotech, and contract research laboratories that need a 500 mL bottle format for routine culture maintenance, feasibility studies, and method transfer.

Store CCM-SC-0018 at 2–8 °C protected from light. Do not freeze unless the receiving laboratory has validated freezing for this specific formulation, as freeze-thaw exposure can alter medium performance. The stated shelf life is 12 months when the product is stored unopened under recommended conditions. Handle using aseptic technique in a qualified biosafety cabinet, and minimize repeated warming cycles by aliquoting only when the laboratory’s sterility controls and container compatibility are validated. Inspect the bottle before use for leakage, turbidity, precipitate, or color changes, and equilibrate to the working temperature and CO2 environment according to the cell culture protocol before contact with cells.

CellCultureMedia supplies CCM-SC-0018 for research and bioprocessing use under ISO-aligned quality practices, with lot traceability and specification review available for procurement files. A Certificate of Analysis can be requested to confirm lot-specific release information, including pH, osmolality, endotoxin, sterility-related release criteria, fill size, and expiration dating where applicable. For quality systems, supplier onboarding, or documentation review, see our quality documentation resources or contact the team through request a quote. Standard shipment uses blue ice to support refrigerated transit, and eligible orders include free worldwide shipping. Buyers can request volume pricing, recurring supply planning, and shipment timing aligned with project milestones or standing purchase agreements.