MEM Medium with Phenol Red, L-Glutamine and Earle Salts

Minimum Essential Medium is a widely used classical medium format for adherent mammalian cell culture. This configuration includes phenol red for visual pH indication, 1.0 g/L D-glucose as the carbohydrate source, 2 mM L-glutamine as a nitrogen and energy-related nutrient, and Earle balanced salts for bicarbonate-buffered incubation. It does not include non-essential amino acids, allowing laboratories to control supplementation when a defined comparison or established internal protocol requires that omission.

CCM-CM-0023 is supplied as a ready-to-use liquid in a 500 mL bottle. The product is formulated without animal-derived components; serum, when required, is added separately by the user. Source specifications indicate use with 10% fetal bovine serum for representative workflows. Key release specifications include pH 7.0–7.4, osmolality 260–320 mOsmol/kg, endotoxin ≤1 EU/mL, and filter-sterilized liquid form. For related formulations, visit the classical media category.

• Routine culture of fibroblasts under established MEM-based protocols.
• Culture of primary rat astrocytes and other research cell models that require MEM with Earle balanced salts.
• Adherent mammalian cell expansion at 37 °C in a humidified incubator with 5–10% CO2, following local SOPs for seeding density, serum supplementation, and medium-exchange frequency.
• Comparative studies where phenol red is required for visual monitoring of culture pH and non-essential amino acids are intentionally excluded from the medium formulation.
• Academic, biotechnology, and bioprocess R&D workflows requiring procurement-ready specifications for pH, osmolality, endotoxin, sterility, shelf life, storage, and shipping conditions.

Store CCM-CM-0023 at 2–8 °C and protect the bottle from light to maintain performance through the stated 12-month shelf life. Do not freeze unless validated by your laboratory, because freeze-thaw exposure can alter solute balance and container integrity. Warm only the required volume to working temperature and use aseptic technique when opening, aliquoting, or adding supplements. For use, laboratories typically add 10% fetal bovine serum or an internally validated alternative supplement system. The recommended culture environment is 37 °C with 5–10% CO2, consistent with bicarbonate-buffered media containing Earle salts. Product ships on blue ice to support temperature control during transit.

CellCultureMedia supplies CCM-CM-0023 for research and process-development use with specification-focused lot documentation. Manufacturing and quality controls are ISO-aligned and include filtration-based sterility processing, pH testing, osmolality testing, and endotoxin release criteria as listed above. A Certificate of Analysis can be requested for lot traceability, incoming inspection, and quality review through the quality documentation page. The standard unit size is 500 mL, with a listed price of $48.61 per bottle. For standing orders, multi-bottle requirements, or project-based procurement, submit details through request a quote. Free worldwide shipping is available, with cold-chain packaging selected according to destination and order profile.