MEM Alpha Modified Medium, 500 mL

CCM-CM-0024 is a ready-to-use liquid MEM Alpha modified medium designed for mammalian cell culture systems that require enriched amino acid and energy-substrate support. This 500 mL bottle is supplied with phenol red as a visual pH indicator, 1.0 g/L D-glucose for carbohydrate input, 2 mM L-glutamine, 0.11 g/L sodium pyruvate, and MEM non-essential amino acids. The product is configured as animal-origin-free before any user-selected serum supplementation and is commonly paired with 10% FBS when required by established laboratory protocols.

Key release specifications include pH 7.0–7.4, osmolality 260–320 mOsmol/kg, endotoxin not more than 1 EU/mL, and sterile-filtered liquid format. It is intended for incubator conditions of 37°C with 5–10% CO2, depending on the cell system and laboratory method. For related classical nutrient formulations, procurement teams can compare options in the classical media category. The balanced composition supports routine R&D use while giving process teams a defined specification set for incoming material review, lot qualification, and repeat purchasing.

• Routine culture of fibroblasts and other adherent mammalian cells requiring MEM Alpha modified nutrient support.
• Culture of primary rat astrocytes and similar primary-cell workflows where the laboratory protocol specifies MEM Alpha modified medium with serum supplementation.
• Scientific and basic research experiments requiring phenol red monitoring, sodium pyruvate, L-glutamine, and MEM non-essential amino acids in a single liquid medium.
• Small-batch method development, cell expansion studies, and academic laboratory use where a sterile-filtered 500 mL bottle size reduces open-container handling.
• Procurement standardization for teams that need documented pH, osmolality, endotoxin, sterility, storage, and shelf-life parameters for the same CCM-* item number across repeat orders.

Store CCM-CM-0024 at 2–8°C protected from light. Do not expose the bottle to repeated warming cycles or extended room-temperature holding, as L-glutamine and phenol red-containing media should be handled consistently to preserve formulation performance. The stated shelf life is 12 months when the product is kept unopened under recommended conditions. Shipments are packed on blue ice to maintain cold-chain handling during transit. Before use, equilibrate only the required volume, mix gently without foaming, and apply aseptic technique after opening. Confirm that the medium remains clear and within expected color range before adding serum or other protocol-specific supplements.

CellCultureMedia supplies CCM-CM-0024 for research and bioprocess development environments that require traceable specifications and procurement-ready documentation. Manufacturing and quality review follow ISO-aligned practices, with sterility, endotoxin, pH, osmolality, appearance, and formulation attributes controlled at lot release. A Certificate of Analysis is available on request through the quality documentation team. For recurring orders, multi-lot planning, or volume pricing, submit requirements through request a quote. Standard 500 mL units ship with free worldwide shipping where service is available, helping purchasing teams consolidate international sourcing without adding freight complexity to the unit-cost review.