IMDM Medium with HEPES and Pyruvate

CCM-CM-0030 is a ready-to-use liquid IMDM medium for classical cell culture applications requiring a nutrient-rich formulation and buffered handling outside the incubator. The medium contains phenol red for visual pH indication, L-glutamine as a nitrogen and energy source, 0.11 g/L sodium pyruvate, HEPES buffer, and 4.5 g/L D-glucose. It is supplied without β-mercaptoethanol and without α-thioglycerol, which is useful for laboratories that prefer to qualify those additives independently by lot, concentration, or experimental design.

This 500 mL format is specified for work with Jurkat, COS-7, macrophage, and similar mammalian cell culture systems after local suitability testing. The medium is intended to be used with 10% fetal bovine serum for the referenced workflows. Product specifications include pH 6.9-7.3, osmolality 270-310 mOsmol/kg, endotoxin ≤1 EU/mL, and filter-sterilized manufacture. For related formulations, procurement teams can compare options in our classical media category.

• Routine maintenance and expansion of validated Jurkat, COS-7, macrophage, and other mammalian cell cultures requiring an enriched IMDM base.
• Serum-containing research workflows in which the user adds 10% fetal bovine serum and qualifies the complete medium in-house.
• Culture conditions of 37°C with 5-10% CO2, using HEPES buffering to improve pH stability during routine manipulation and sampling.
• Protocols requiring phenol red, L-glutamine, sodium pyruvate, and high-glucose composition in one liquid, ready-to-supplement medium.
• Experiments where β-mercaptoethanol and α-thioglycerol must be omitted from the base medium and introduced separately only if needed.
• Academic, biotech, and bioprocess development laboratories seeking a documented, animal-origin-free medium component for basic research workflows.

Store CCM-CM-0030 at 2-8°C protected from light. The shelf life is 12 months when the bottle remains unopened and is held under the stated conditions. The product ships on blue ice to maintain cold-chain integrity; equilibrate gently before use and handle aseptically after opening. Do not use the medium if the container is compromised or if appearance is inconsistent with a clear liquid medium containing phenol red. Warm only the volume required for immediate use and record lot number, opening date, and serum supplementation details in the batch record.

CellCultureMedia supplies CCM-CM-0030 as a procurement-ready research reagent manufactured under ISO-aligned quality practices, with filter sterility, pH, osmolality, endotoxin, and appearance testing assigned by lot. A Certificate of Analysis is available on request, and additional documentation can be coordinated through our quality documentation team. The listed 500 mL price is USD 48.61, with free worldwide shipping where service is available. For standing orders, multi-bottle purchasing, or project-specific supply planning, contact CellCultureMedia through the request quote form and include CCM-CM-0030, required quantity, destination, and delivery schedule.