High-Glucose DMEM with Phenol Red

CCM-CM-0009 is a classical high-glucose Dulbecco's Modified Eagle Medium configuration for laboratories that need a defined base medium with phenol red but without L-glutamine, sodium pyruvate, and HEPES. The formulation includes 4.5 g/L D-glucose, supporting high-energy culture formats commonly used for adherent mammalian cells. Because glutamine and pyruvate are omitted, process engineers and R&D teams can select the grade, concentration, and timing of these supplements according to internal protocols, stability requirements, or experimental design.

The product is supplied as a ready-to-use liquid in a 500 mL size and is classified as an animal-origin-free medium base. For the stated use profile, the medium requires supplementation with 10% fetal bovine serum or an internally qualified alternative before culture. Specifications include pH 7.0-7.4, osmolality 320-350 mOsmol/kg, endotoxin not more than 1 EU/mL, and filter-sterilized processing. This item belongs to our classical media portfolio and is priced at USD 48.61 per 500 mL bottle before any account-specific quotation terms.

• Routine expansion and maintenance of adherent mammalian cells, including fibroblasts and smooth muscle cells, after completion with qualified serum and any protocol-specific supplements.
• Research and basic science workflows requiring high-glucose DMEM with phenol red for visual pH indication during standard incubation.
• Culture conditions at 37 degrees C with 5-10% CO2, where bicarbonate-based buffering is used and HEPES is intentionally excluded.
• Comparative media studies in which L-glutamine, sodium pyruvate, serum source, or supplement timing must be controlled independently by the user.
• Procurement standardization for academic, biotech, and bioprocess development groups that need consistent 500 mL liquid media lots with documented pH, osmolality, sterility, and endotoxin criteria.

Store CCM-CM-0009 at 2-8 degrees C protected from light. Do not freeze unless a validated internal procedure supports it, as freezing can affect salts, indicators, and other medium components. The stated shelf life is 12 months when unopened and stored under the recommended conditions. Upon receipt, inspect the bottle and confirm that the blue-ice shipment remained cold. Use aseptic technique for all dispensing steps, minimize repeated warming cycles, and return unused medium promptly to refrigerated storage. Add serum, L-glutamine, sodium pyruvate, HEPES, or other supplements only after confirming compatibility with the receiving cell system and the laboratory's approved protocol.

CellCultureMedia supplies CCM-CM-0009 for research and bioprocess development environments that require lot-level traceability and procurement-ready documentation. Manufacturing and release controls are aligned with ISO-style quality practices, and each lot is tested against defined acceptance criteria for appearance, pH, osmolality, sterility by filtration process controls, and endotoxin limit. A Certificate of Analysis can be requested through our quality documentation channel for lot confirmation, audit files, or incoming material review.

For recurring demand, multi-bottle orders, or standing supply requirements, procurement teams can submit specifications through request a quote. We can quote bulk pricing, planned replenishment, and shipping coordination based on destination and quantity. Standard handling is blue-ice shipment, and free worldwide shipping is available where logistics coverage and import conditions allow.