Ham's F-12 Nutrient Medium, 500 mL

CCM-CM-0021 is a Ham's F-12 Nutrient Medium for classical cell culture applications requiring a chemically defined nutrient background with phenol red pH indicator and L-glutamine already included. The formulation is supplied as a ready-to-use liquid and is suitable for workflows involving CHO cells, chondrocytes, epithelial cells, and comparable adherent or suspension systems where Ham's F-12 chemistry is specified. It contains 1.8 g/L D-glucose and 1 mM L-glutamine, supporting routine culture setup without separate glutamine addition at the point of use.

The product is specified as a base medium that contains no animal-derived components before user supplementation. For the listed use profile, supplementation with 10% fetal bovine serum is required unless an internally validated alternative supplement strategy is used. Typical culture conditions are 37 °C in a 5-10% CO2 incubator. Release specifications include pH 7.5-7.9, osmolality 280-320 mOsmol/kg, endotoxin not more than 1 EU/mL, and sterility by filtration. For related formulations, see our classical media category.

• Routine expansion and maintenance of CHO cell cultures where Ham's F-12 nutrient composition is part of an established protocol.
• Culture of epithelial cell models requiring phenol red for visual pH monitoring during standard incubator operation.
• Chondrocyte culture workflows using serum-supplemented classical medium in basic research environments.
• Comparative media screening, raw material qualification, and process development studies where pH, osmolality, endotoxin, and sterility specifications must be documented for each lot.
• Academic, biotechnology, and biopharma R&D programs that need a 500 mL liquid format for controlled consumption, reduced open-container time, and straightforward inventory rotation.

Because this medium is supplied with L-glutamine, users should confirm compatibility with in-house hold-time limits, feeding strategies, and incubation schedules. The phenol red indicator supports rapid visual assessment of culture environment changes, while formal acceptance should remain based on validated analytical and cell-based criteria.

Store CCM-CM-0021 at 2-8 °C protected from light. Do not freeze, as freezing can affect component solubility and container integrity. The product is shipped on blue ice to maintain cold-chain conditions during transit; on receipt, inspect the bottle, verify the label, record the lot number, and transfer promptly to refrigerated storage. Shelf life is 12 months when the unopened product is stored under the stated conditions. Use aseptic technique after opening, minimize repeated warming cycles, and avoid extended exposure to ambient light. If the medium shows turbidity, unexpected color shift, precipitate, or compromised packaging, quarantine the unit and contact CellCultureMedia before use.

CellCultureMedia supplies CCM-CM-0021 for research and bioprocessing use with procurement-grade traceability and lot documentation. Manufacturing and release controls are aligned with ISO-style quality system practices, including filtration-based sterility control, endotoxin specification, pH verification, and osmolality testing. A Certificate of Analysis can be requested for the applicable lot through our quality documentation process.

The 500 mL pack size is priced from $48.61, with volume pricing available for scheduled supply, multi-lot reservations, and larger purchasing programs. Free worldwide shipping is available where lane and temperature-control requirements can be supported. To confirm current inventory, shipping route, documentation needs, or standing-order options, submit a request through request a quote.