DMEM/F-12 Phenol Red-Free Medium with HEPES

CCM-CM-0019 is a DMEM/F-12 (1:1) classical liquid medium designed for routine mammalian cell culture where a balanced mixture of amino acids, vitamins, salts, glucose, and buffering components is required. The formulation includes 3.15 g/L D-glucose, 2.5 mM L-glutamine, 55 mg/L sodium pyruvate, and HEPES. Phenol red is omitted to reduce colorimetric background in fluorescence, absorbance, and hormone-sensitive experimental designs. For related formulations, visit the classical media category.

The medium is manufactured as an animal-origin-free base formulation and is commonly completed by the end user with 10% fetal bovine serum or another validated supplement package. Key specifications include liquid format, filter sterilization, endotoxin ≤1 EU/mL, pH 7.0-7.4, and osmolality of 290-330 mOsmol/kg. Recommended culture conditions are 37 °C with 5-10% CO2, subject to cell-line-specific optimization. The 500 mL pack size supports R&D, assay development, pilot-scale evaluation, and procurement programs needing lot-traceable medium with defined release criteria.

• Serum-supplemented culture of neuronal, glial, endothelial, and other mammalian cell models requiring DMEM/F-12 nutrient balance.
• Phenol red-free culture workflows where optical background, pH indicator color, or assay readout compatibility must be managed.
• Research cultures using 10% fetal bovine serum, user-qualified growth supplements, or custom supplementation strategies.
• CO2 incubator culture at 37 °C with 5-10% CO2, with HEPES providing additional buffering capacity during handling outside the incubator.
• Comparability studies, media qualification, and small-batch process development where pH, osmolality, endotoxin, and sterility data are reviewed before purchasing.

Store CCM-CM-0019 at 2-8 °C and protect from light. Do not freeze unless a site-specific stability assessment has been completed. The stated shelf life is 12 months when the bottle remains unopened and is stored under recommended conditions. Use aseptic technique after opening, minimize repeated warming cycles, and record the opening date according to internal laboratory procedures. The product ships on blue ice to maintain controlled temperature during transit. Inspect the bottle on receipt for seal integrity, clarity, volume, and labeling before release into cell culture use.

CellCultureMedia supplies CCM-CM-0019 for research and bioprocessing customers that require consistent specifications and procurement-ready documentation. Manufacturing and quality checks are ISO-aligned, with release testing for sterility, pH, osmolality, and endotoxin. A Certificate of Analysis is available on request, and documentation questions can be directed through the quality information page. For standing orders, bulk requirements, or institution-wide pricing, submit details through request a quote. Free worldwide shipping is available on qualified orders, with temperature-managed blue-ice dispatch for this liquid medium.