CD 293 Serum-Free ADCF Medium

CCM-SF-0055 is a ready-to-use liquid serum-free medium designed for 293 suspension culture where defined composition, animal-derived component-free formulation, and low background monitoring are required. The medium is supplied without phenol red to reduce colorimetric interference in optical assays and process analytics. It is formulated with 6.0 g/L D-glucose, 3 mM L-glutamine, and 3 mM L-alanyl-L-glutamine, combining immediately available glutamine with a stabilized dipeptide source that supports longer culture intervals and feed strategy development.

Key release specifications include sterile-filtered liquid format, pH 7.0-7.4, osmolality 270-340 mOsmol/kg, and endotoxin not more than 3 EU/mL. The product is intended for culture at 37°C in 5-10% CO2, consistent with common 293 suspension workflows. Its animal-derived component-free classification helps process engineers reduce undefined inputs during upstream development, comparability studies, and scale-up planning. The 500 mL pack size is suitable for screening, shake flask work, seed train evaluation, and small bioreactor runs before larger procurement commitments.

• Suspension culture of HEK 293F-type cells and comparable 293-derived expression platforms in chemically defined, serum-free conditions.
• Transient transfection process development for recombinant protein, plasmid, and viral vector production workflows where phenol red-free medium is preferred.
• Seed train preparation and expansion studies using animal-derived component-free medium to support more controlled upstream process inputs.
• Media screening, feed timing studies, and osmolality or pH response evaluation in shake flasks, spinner vessels, and bench-scale bioreactors.
• Analytical workflows requiring reduced color interference, including cell growth monitoring, metabolite analysis, and optical readouts.

Store CCM-SF-0055 at 2-8°C protected from light. Do not freeze, and minimize repeated warming cycles to preserve medium performance. Before use, equilibrate the container to the required working temperature under controlled laboratory conditions and mix gently to ensure homogeneity; avoid vigorous shaking that may introduce foam or compromise handling in aseptic operations. The shelf life is 12 months when the unopened container is stored as specified. Use aseptic technique after opening and follow site procedures for hold time, in-use dating, and disposal. The recommended culture condition is 37°C with 5-10% CO2, with final process settings determined by the cell line, vessel, inoculation density, and target expression protocol.

CellCultureMedia supplies CCM-SF-0055 for B2B research and bioprocess procurement with specification-driven documentation and lot traceability. Manufacturing and release controls are ISO-aligned, and a Certificate of Analysis can be requested through our quality documentation team for lot-specific confirmation of sterility, endotoxin, pH, osmolality, and appearance criteria. Shipments are packed on blue ice, and free worldwide shipping is available for this item. For standing supply, multi-pack orders, or project-based sourcing, buyers can request a formal quote with pricing, availability, shipping lane details, and documentation requirements for internal approval.