NK Cell Serum-Free Medium, Animal-Origin-Free
CCM-SF-0069 is a liquid, animal-origin-free, serum-free medium formulated for NK cell culture workflows. Supplied in a 1 L format, it is designed for incubation at 37 °C with 4–6% CO2 and does not require serum supplementation. The formulation includes D-glucose, L-glutamine, alanyl-glutamine, phenol red, and sodium bicarbonate for controlled culture conditions. CellCultureMedia offers free worldwide shipping and quote-based procurement options for research groups, process development teams, and manufacturing-adjacent laboratories.
- ✓ Free worldwide shipping
- ✓ ISO-aligned manufacturing
- ✓ Certificate of Analysis on request
- ✓ Bulk & custom volumes quoted on request
Overview
CCM-SF-0069 is a ready-to-use serum-free medium for NK cell culture in research, development, and bioprocessing environments where defined composition and animal-origin-free classification are procurement priorities. The medium is supplied as a liquid and is intended for standard humidified CO2 incubator culture at 37 °C with 4–6% CO2. It is part of CellCultureMedia’s serum-free media portfolio for laboratories seeking consistent lot documentation, simplified sourcing, and scalable ordering.
The specification profile is built around controlled culture parameters: pH 7.0–7.4, osmolality 280–320 mOsmol/kg, endotoxin ≤1 EU/mL, and sterility achieved by filtration. The product requires no serum addition, reducing variability associated with undefined supplements. The formulation contains D-glucose as an energy source, L-glutamine and alanyl-glutamine as nitrogen sources, phenol red as a visual pH indicator, and sodium bicarbonate for CO2-buffered culture systems. These characteristics make the medium suitable for teams evaluating NK cell expansion, culture consistency, and process comparability under serum-free conditions.
Applications
- Serum-free NK cell culture for R&D laboratories and process development groups requiring animal-origin-free media inputs.
- Evaluation of NK cell growth, viability, and culture performance under defined medium conditions without added serum.
- Development of closed or semi-closed cell culture workflows where documented sterility, endotoxin, pH, and osmolality specifications are needed for supplier qualification.
- Comparability studies across medium lots, culture vessels, and CO2 incubation settings using a liquid format that is ready for routine laboratory use.
- Procurement programs that require a 1 L unit size, transparent specifications, and supplier documentation accessible through request-quote workflows.
Storage and Handling
Store CCM-SF-0069 at 2–8 °C protected from light. Do not freeze unless a validated internal procedure has established that performance is maintained after freeze-thaw exposure. The stated shelf life is 12 months when stored under recommended conditions. Inspect the bottle before use for container integrity, clarity, and expiration status. Handle aseptically in a suitable biosafety cabinet or clean workflow to preserve sterility after opening. The medium is shipped on blue ice to maintain refrigerated conditions during transit; upon receipt, transfer promptly to 2–8 °C storage and minimize exposure to room temperature and direct light during routine use.
Quality and Documentation
CellCultureMedia supplies CCM-SF-0069 for professional cell culture procurement with specification-led documentation. Manufacturing and quality controls are ISO-aligned, and a lot-specific Certificate of Analysis can be provided on request, including relevant release parameters such as sterility, endotoxin, pH, osmolality, appearance, and expiration dating where applicable. Additional supplier and quality information is available through the quality page. The listed 1 L price is $643.06, with free worldwide shipping available for eligible orders. For recurring supply, project stocking, or larger-volume requirements, procurement teams may request a formal quotation with volume pricing, shipping details, and documentation needs consolidated for purchasing review.
Specifications
Formulation Highlights
Contains (+)
[+]D-葡萄糖 [+]L-谷氨酰胺 [+]丙氨酰谷氨酰胺[+]酚红 [+]碳酸氢钠
Storage & Handling
Storage: 2~8 ℃,避光. Shelf life: 12个月. Ship cold-chain where required.
Quality & Documentation
Manufactured under ISO-aligned quality management systems. Full documentation — Certificate of Analysis (CoA), Material Safety Data Sheet (MSDS), formulation summary, and stability data — is available on request. Email [email protected] with the SKU.