0.4% Trypan Blue Solution

CCM-RG-0021 is a 0.4% Trypan Blue Solution supplied as a ready-to-use liquid reagent for cell culture-associated counting workflows. Trypan blue is commonly used as an exclusion dye: cells with compromised membrane integrity take up the dye, while intact cells generally exclude it. This supports manual hemocytometer counting, automated cell counter sample preparation where compatible, and standard in-process checks during R&D, media development, seed-train monitoring, and routine culture maintenance.

The product is sterile filtered and supplied in a 100 mL format for laboratory and process-development use. Key specifications include liquid form, osmolality of 290–340 mOsmol/kg, storage at 2–30 °C, ambient shipping conditions, and a stated shelf life of 36 months when stored as directed. Procurement teams can source this reagent within the broader cell culture reagents portfolio to consolidate routine consumables, reduce vendor fragmentation, and standardize documentation expectations across research groups.

• Routine cell counting using dye-exclusion methods in academic, biotech, and biopharma laboratories.
• Live and nonviable cell discrimination during passaging, culture expansion, transfection optimization, clone screening, and media-comparison studies.
• In-process sample checks for suspension and adherent cell culture workflows after appropriate cell detachment or sample preparation.
• Compatibility evaluation with automated cell counting instruments that specify 0.4% trypan blue input; users should confirm dilution ratios, counting chamber requirements, and instrument settings internally.
• Standardized reagent supply for cell culture additive inventories, QC support labs, R&D teams, and process engineering groups requiring defined osmolality and sterile-filtered liquid format.

Store CCM-RG-0021 at 2–30 °C in the original closed container and protect it from unnecessary contamination during repeated use. The product ships at ambient temperature, supporting simpler receiving workflows and reducing cold-chain handling requirements. Allow the bottle to equilibrate to room temperature before use if stored under cooler conditions, and mix gently before aliquoting. Use aseptic technique when withdrawing reagent, avoid returning unused liquid to the bottle, and record opening dates according to your laboratory quality system. The assigned shelf life is 36 months when stored according to specification.

CellCultureMedia supplies CCM-RG-0021 for research, cell culture, and bioprocessing support applications under ISO-aligned quality and documentation practices. Lot-specific documentation, including a Certificate of Analysis when available, can be requested through quality support. Typical release information may include identity, appearance, sterility status by filtration process, osmolality range, shelf-life assignment, and storage conditions. For bulk demand, scheduled replenishment, or site-level pricing, submit requirements through request a quote. Free worldwide shipping is available, and orders are handled with packaging appropriate for ambient transport of this liquid reagent.