0.05% Trypsin-EDTA Solution, 500 mL

CCM-RG-0045 is a 0.05% Trypsin-EDTA cell dissociation reagent formulated for controlled detachment of adherent cell monolayers from standard culture vessels. The formulation combines 0.5 g/L trypsin with 0.2 g/L EDTA·4Na, supporting proteolytic and chelation-driven release of cells from extracellular attachment points. Phenol red is included as a visual pH indicator for routine handling checks. The solution is supplied as a liquid, filter-sterilized product in a 500 mL format for laboratories and process-development teams standardizing passaging operations.

This reagent is classified as animal-origin and is intended for cell culture research, assay preparation, seed-train work, and bioprocess development where a defined trypsin-EDTA concentration is required. Release specifications include pH 7.2–8.0 and osmolality of 270–320 mOsmol/kg, supporting compatibility with common mammalian cell culture environments. For related dissociation buffers, balanced salt solutions, and media supplements, review our cell culture reagents portfolio.

• Routine detachment and passaging of adherent mammalian cell cultures in flasks, dishes, roller bottles, and multiwell plates.
• Preparation of single-cell suspensions before cell counting, viability assessment, plating, expansion, or cryopreservation workflows.
• R&D and process-development studies that require a mild 0.05% trypsin concentration with EDTA-assisted dissociation.
• Scale-up support for seed-train operations where consistent detachment timing, lot documentation, and procurement continuity are important.
• Laboratory workflows where phenol red provides quick visual confirmation that the reagent has not undergone obvious pH shift during routine use.

For optimal results, users should validate exposure time, neutralization method, and wash steps for each cell type and vessel format. Sensitive lines may require shorter contact times, pre-rinsing to reduce serum carryover, or alternative dissociation conditions selected during internal method qualification.

Store CCM-RG-0045 at -30 to -5 °C protected from light. The product ships on dry ice and is specified with a 24-month shelf life when stored as recommended and kept sealed. Thaw at controlled temperature, mix gently after thawing, and avoid repeated freeze-thaw cycles by aliquoting into sterile, validated containers if required by your workflow. Use aseptic technique after opening and keep the bottle closed when not in use. Do not use if the container is compromised, the solution shows unexpected turbidity, or internal acceptance checks indicate out-of-range performance.

CellCultureMedia supplies CCM-RG-0045 for procurement teams that require traceable lot control, specification visibility, and dependable replenishment. Manufacturing and release workflows are ISO-aligned, and the product is filter-sterilized for cell culture reagent use. A Certificate of Analysis is available on request and may include lot-specific confirmation of sterility, pH, osmolality, shelf life, and other released parameters. Endotoxin specification is not listed in the supplied release parameters; if endotoxin reporting is required for your workflow, request lot-specific data before purchase.

For documentation support, visit our quality information page or submit a volume inquiry through request a quote. Standard ordering is available for the 500 mL size, with bulk pricing options for qualified recurring demand. Free worldwide shipping is available, with dry-ice packaging selected to preserve frozen condition during transit.