Insect Cell Serum-Free Feed, ADCF

CCM-SC-0004 is a ready-to-use serum-free feed for insect cell culture, supplied as a liquid in a 500 mL bottle. It is designed for SF9 and HighFive cell systems used in research, upstream process development, and biopharmaceutical production workflows. The formulation is animal-derived component-free and serum-free, supporting defined culture inputs and simplified raw-material review for regulated laboratory and manufacturing environments. It includes D-glucose as a listed component and excludes phenol red and L-glutamine, which can be useful when visual pH indicator dyes are not desired or when glutamine supplementation is managed separately by the process owner.

The product is sterile-filtered and specified with an endotoxin limit of ≤20 EU/mL. Source culture parameters list use at 37 °C with 5–10% CO2, and users should confirm final incubation set points against their validated insect cell platform, clone requirements, and process history. The material is part of the serum-free media portfolio available through CellCultureMedia cell culture media categories, with specification support for procurement, R&D, and process engineering teams.

• Serum-free feeding of SF9 insect cells in suspension or process-development culture formats.
• Serum-free feeding of HighFive insect cells where animal-derived component-free raw materials are required.
• Upstream development for recombinant protein, vector, vaccine, and other biopharmaceutical production workflows using insect cell expression systems.
• Phenol-red-free culture strategies requiring reduced background color interference for optical monitoring, imaging, or assay compatibility.
• Processes where L-glutamine is added separately, substituted, or controlled through a defined supplementation plan.
• Scale-up studies requiring a liquid, sterile-filtered feed with documented shelf life, storage conditions, and endotoxin specification.

Store CCM-SC-0004 at 2–8 °C protected from light. The product is supplied as a sterile-filtered liquid and should be handled using aseptic technique after opening. Do not use if the bottle, closure, or liquid appearance indicates damage or compromised integrity. The stated shelf life is 12 months when stored under the recommended refrigerated and light-protected conditions. The product ships on blue ice to help maintain refrigerated transit conditions; upon receipt, inspect packaging, record arrival condition according to your site procedure, and transfer the bottle promptly to controlled 2–8 °C storage. Avoid repeated warming cycles and minimize open-container time during aliquoting or feeding operations.

CellCultureMedia supplies CCM-SC-0004 for research and bioprocessing use with procurement-ready documentation support. Manufacturing and quality release are aligned with ISO-style quality system expectations for cell culture reagents, including lot traceability and specification review. A Certificate of Analysis can be requested through the CellCultureMedia quality documentation channel, and available lot documentation may include sterility status, endotoxin specification, shelf life, storage condition, and product identity information. For volume planning, standing orders, or project-based sourcing, submit requirements through request a quote. Published 500 mL pricing starts at $643.06, and qualified orders can include free worldwide shipping depending on destination, pack-out, and order profile.