Sf9 Insect Cell Serum-Free Medium

CCM-SC-0001 is a ready-to-use liquid serum-free medium designed for insect-cell culture workflows that require animal-derived component-free composition, defined supplementation, and reduced lot-to-lot variability. The formulation includes D-glucose as a carbohydrate source and 1.5 mM L-glutamine to support protein and nucleotide metabolism during routine expansion and production-stage culture. Phenol red is excluded, which can be useful when optical readouts, color-sensitive assays, or downstream analytical methods benefit from a dye-free background.

Key release specifications include sterile-filtered processing, endotoxin not more than 10 EU/mL, pH 6.0-6.4, and osmolality 360-440 mOsmol/kg. The recommended culture environment is 27-28°C in an air-regulated, non-CO2 atmosphere. While the product title is aligned to Sf9 insect-cell use, buyers evaluating HEK293-family suspension workflows should confirm cell-line performance during internal qualification. For controlled purchasing, the 500 mL pack is listed at USD 241.67 and can be referenced by CellCultureMedia SKU CCM-SC-0001.

• Serum-free suspension expansion of Sf9 insect cells under non-CO2 incubation at 27-28°C.
• Process development where animal-derived component-free media are required to simplify raw-material risk assessment.
• Recombinant protein, antigen, vector, and biologics research workflows that use insect-cell expression platforms.
• Assay development or analytical sampling where absence of phenol red may reduce color interference.
• Media screening, scale-down model qualification, and raw-material comparison studies for R&D and process engineering groups.

Store unopened bottles at 2-8°C protected from light. Do not freeze unless validated by the user, and minimize repeated warming cycles to help preserve defined medium performance. The stated shelf life is 12 months when stored as recommended and handled using aseptic technique. Before use, equilibrate only the required volume to the intended culture temperature and mix gently; avoid vigorous shaking that can introduce foam or excessive gas exchange. Inspect the liquid for clarity, container integrity, and expiration date before release to culture operations.

CellCultureMedia supplies CCM-SC-0001 for research, development, and bioprocessing use with procurement-ready specifications for sterility, endotoxin, pH, osmolality, format, shelf life, storage, and formulation exclusions. Manufacturing and release controls are aligned with ISO-style quality management expectations for cell-culture raw materials; learn more at quality and documentation. A Certificate of Analysis can be requested for lot review, vendor qualification, or incoming QC files. For standing orders, multi-bottle requirements, or bulk pack planning, submit a request through request a quote. Free worldwide shipping is available where import and courier conditions allow.