Serum-Free CHO Transient Expression Medium

CCM-SF-0007 is designed for transient recombinant protein expression in suspension-adapted CHO platforms where defined composition, reduced animal-derived risk, and lot-to-lot consistency are procurement priorities. The formulation is serum-free and animal origin-free, supplied as a ready-to-use liquid for culture at 37 degrees C in 5–10% CO2. Phenol red is included for visual pH indication during routine handling, while D-glucose and 6 mM L-glutamine support the metabolic demand associated with transient expression campaigns.

Key release specifications include pH 7.0–7.4, osmolality 270–340 mOsmol/kg, sterile-filtered processing, and endotoxin not more than 3 EU/mL. The product is suitable for laboratories evaluating expression conditions, feed timing, DNA-to-reagent ratios, and small- to mid-scale shake flask or controlled bioreactor workflows. It is supplied under CellCultureMedia SKU CCM-SF-0007 in a 500 mL bottle, with a listed price of $319.44 before any negotiated volume terms, standing agreements, or project-specific quotations.

• Transient expression in suspension CHO cells used for recombinant protein, antibody fragment, fusion protein, and process-development studies.
• Serum-free adaptation and performance screening where an animal origin-free medium is required by internal sourcing or process control standards.
• Early-stage bioprocess development, including shake flask screening, seed train evaluation, and transfer into controlled stirred-tank or single-use systems.
• Comparability studies where defined pH, osmolality, endotoxin, sterility, and shelf-life data are needed for materials qualification.
• Academic, biotechnology, and biopharma R&D groups that require a ready-to-use CHO transient expression medium with phenol red and L-glutamine already included.

Store CCM-SF-0007 at 2–8 degrees C protected from light. Do not use after the assigned 12-month shelf life, and avoid repeated temperature excursions during receipt, aliquoting, or daily use. The product is shipped on blue ice; upon arrival, inspect the bottle, label, and temperature condition before placing it into refrigerated storage. Use aseptic technique after opening, keep the cap closed when not in use, and pre-warm only the volume required for the day’s culture operation. Because the formulation contains L-glutamine, minimize unnecessary warming time to help maintain formulation consistency.

CellCultureMedia supplies CCM-SF-0007 for research and bioprocessing environments that require specification-driven sourcing and traceable documentation. Manufacturing and release controls are aligned with ISO-style quality practices, and lot-specific documentation can be requested through the quality documentation page. Certificates of Analysis typically confirm identity, appearance, pH, osmolality, sterility status, endotoxin specification, storage conditions, and expiry dating. For multi-bottle orders, scheduled replenishment, or bulk purchasing, submit requirements through request a quote. Free worldwide shipping is available for eligible destinations, with cold-chain packaging selected to support refrigerated liquid media transit.