Serum-Free CHO Powder Medium with HT Supplement

CCM-SF-0006 is a defined serum-free medium powder for suspension or adapted CHO cell culture programs that require an animal origin-free formulation. The product is configured with phenol red for visual pH indication, D-glucose as the principal carbohydrate source, and HT supplement, commonly used to support nucleotide salvage pathways in suitable CHO host systems. L-glutamine is intentionally excluded, allowing process engineers to add a preferred glutamine source, stabilized glutamine substitute, or feed strategy at the concentration required by the project.

The 10 L pack supports laboratory scale screening, seed train evaluation, and small-batch upstream process work. Reported specifications include powder form, serum-free classification, animal origin-free composition, endotoxin not more than 3 EU/mL, osmolality 250-290 mOsmol/kg, pH 4.2-4.8, and 24-month shelf life when stored as specified. Standard culture conditions are 37 °C with 5-10% CO₂, subject to the validated requirements of the user’s cell line and equipment. For related formulations, see the CellCultureMedia serum-free media category.

• Serum-free expansion of CHO K1, CHO-S, CHO-GS, and CHO DG44 cells used in R&D and upstream bioprocess development.
• Animal origin-free workflows where procurement teams require reduced reliance on serum or undefined animal-derived inputs.
• Cell line adaptation, medium screening, and feed-strategy studies where L-glutamine is added separately for tighter formulation control.
• Biopharmaceutical protein expression and vaccine-process development programs requiring documented media components and lot traceability.
• Seed-train and shake flask or small bioreactor studies operated near 37 °C and 5-10% CO₂, with osmolality and pH monitored after reconstitution and supplementation.

Store the unopened powder at 2-8 °C protected from light. The material ships on blue ice or equivalent cold packs to support temperature-controlled delivery; avoid repeated warming, moisture exposure, or prolonged opening of the container. Reconstitute using validated water quality, mixing sequence, filtration, and supplementation procedures established by the user’s laboratory. Because this formulation does not include L-glutamine, add the selected glutamine component only after confirming compatibility with the intended culture duration, feed plan, and analytical controls. Shelf life is 24 months from manufacture when maintained under the specified storage conditions.

CCM-SF-0006 is supplied for cell culture research and bioprocessing under ISO-aligned manufacturing and release practices. Lot-specific documentation is available on request, including a Certificate of Analysis where applicable, to support internal material qualification, incoming QC, and supplier records. Key release attributes include sterility control, endotoxin specification, osmolality, pH, appearance, and traceable lot identification. CellCultureMedia provides free worldwide shipping for this item and can support scheduled procurement, standing requirements, and volume inquiries through quotation review. Additional information on documentation practices is available from our quality resources.