Schneider’s Drosophila Cell Medium, No Phenol Red

Schneider’s Drosophila cell medium is a classical insect-cell formulation used for maintaining and expanding fruit fly cell cultures in academic, biotechnology, and contract research laboratories. CCM-CM-0035 is supplied as a ready-to-use liquid medium that is sterile-filtered and formulated without phenol red, helping laboratories reduce background color interference in assays where visual or optical readouts are important. The formulation includes 2.0 g/L D-glucose as a carbohydrate source and 350 mg/L L-glutamine as a nitrogen source for routine culture workflows.

The product specification lists pH at 6.2–6.8 and osmolality at 300–360 mOsmol/kg, supporting controlled incoming-quality review by procurement and process teams. Endotoxin is specified at ≤1 EU/mL, sterility is achieved by filtration, and shelf life is 12 months when stored as directed. The medium is categorized as animal-origin-free; users may add serum such as 10% FBS only when required by an established protocol. For adjacent formulations and pack formats, see our classical media category.

• Routine culture of Drosophila-derived cells for basic research, screening support, recombinant protein expression studies, and insect cell method development.
• Phenol-red-free assay environments where reduced color contribution from the medium is preferred for absorbance, fluorescence, imaging, or indicator-sensitive workflows.
• Serum-supplemented culture systems, including protocols that specify 10% FBS addition after receipt; supplementation should be validated by the user for the relevant cell line and passage history.
• Research-scale process development requiring defined release specifications for pH, osmolality, endotoxin, sterility, shelf life, storage, and cold-chain transport.
• Laboratories standardizing procurement around 500 mL liquid packs with blue-ice shipment, controlled storage at 2–8 °C, and documentation suitable for internal material qualification.

Incubation parameters should follow the cell line owner’s protocol and internal method validation. The supplied specification references 37 °C with 5–10% CO2; many Drosophila cell workflows use cell-line-specific conditions, so process engineers should confirm temperature, atmosphere, seeding density, and media-change interval before scaling.

Store CCM-CM-0035 at 2–8 °C protected from light. Do not freeze unless your internal validation supports freeze-thaw handling for this specific medium, as freezing may affect salts, amino acids, or other formulation components. Before use, equilibrate only the required volume, mix gently, and use aseptic technique in a qualified biosafety cabinet or equivalent controlled environment. Inspect the bottle before use for container integrity, turbidity, precipitate, or unexpected color change. The assigned shelf life is 12 months from manufacture when the product remains unopened and stored under the recommended refrigerated, light-protected conditions.

CellCultureMedia supplies CCM-CM-0035 for research and bioprocessing customers that require lot traceability, defined specifications, and procurement-ready documentation. Manufacturing and release controls are ISO-aligned, with sterility, endotoxin, pH, osmolality, format, storage, and shelf-life information available for material review. A Certificate of Analysis can be provided on request, and additional quality support is available through our quality documentation portal. Standard 500 mL packs ship on blue ice with free worldwide shipping where applicable. For institutional pricing, standing orders, or multi-bottle demand planning, submit details through request a quote.