Reduced-Serum MEM with Phenol Red and Pyruvate

CCM-SF-0065 is a reduced-serum MEM format for laboratories that need the buffering, indicator, energy-source, and amino acid profile of MEM while lowering routine FBS input to 1-5%. The formulation includes phenol red for visual pH monitoring, 1.0 g/L D-glucose as a carbohydrate source, 0.11 g/L sodium pyruvate, and MEM non-essential amino acids. L-glutamine is intentionally omitted, allowing users to add fresh glutamine or a preferred stable glutamine source according to internal protocols and cell-line requirements.

The medium is supplied as a liquid, sterile-filtered product in a 500 mL size. Key release specifications include pH 7.0-7.4, osmolality 280-330 mOsmol/kg, and endotoxin not more than 1 EU/mL. The source classification is animal origin because the intended reduced-serum workflow uses FBS supplementation. This product fits procurement programs seeking defined lot documentation, controlled cold-chain handling, and reduced-serum options within the serum-free and reduced-serum media category.

• Reduced-serum maintenance or experimental culture of fibroblasts when a MEM background and 1-5% FBS are appropriate for the laboratory method.
• Primary rat astrocyte culture models and other adherent research cell systems requiring MEM non-essential amino acids and sodium pyruvate.
• Media optimization studies comparing standard serum concentration against reduced-serum conditions while keeping glucose, pyruvate, and indicator chemistry consistent.
• R&D screening workflows where phenol red is useful for visual monitoring of medium color during 37 °C incubation in 5-10% CO2.
• Process-development or academic core facility use where L-glutamine omission is preferred for flexible supplementation immediately before use.

Store CCM-SF-0065 at 2-8 °C protected from light. Do not freeze the bottle, and minimize repeated warming to room temperature to maintain consistency over the stated 12-month shelf life. The product is shipped on blue ice and should be transferred to refrigerated storage upon receipt. Use aseptic technique after opening, record open-bottle dating according to site procedures, and add L-glutamine or other supplements only after confirming compatibility with the target cells, serum level, and incubation conditions.

CellCultureMedia supplies CCM-SF-0065 for scientific and basic research workflows under ISO-aligned quality practices with lot-level traceability. A Certificate of Analysis can be requested to confirm sterility, endotoxin, pH, osmolality, shelf-life, and release details for the delivered lot. Procurement teams can request quotations for standing orders, consolidated shipments, or larger volume planning through our quote request page. Additional information on documentation, release controls, and supplier qualification support is available through CellCultureMedia quality resources. Free worldwide shipping is offered where lane and temperature-control requirements can be met.