Reduced Serum Medium, Phenol Red-Free, ADCF

This reduced serum medium is designed for laboratories that need a lower-background culture environment without phenol red and without animal-derived components. The liquid formulation supports workflows where serum concentration, colorimetric interference, and animal-origin inputs must be managed carefully during experimental setup, cell handling, or reagent-complexing steps. Phenol red is omitted to reduce potential assay interference in optical, fluorescence, and hormone-sensitive culture systems. The inclusion of L-glutamine supports nitrogen and energy metabolism, sodium pyruvate provides an additional carbon source, and HEPES improves buffering stability during manipulations outside a fully equilibrated CO2 incubator.

Key release specifications for CCM-RG-0015 include pH 7.0–7.4, osmolality 280–320 mOsmol/kg, endotoxin ≤1 EU/mL, liquid form, animal-origin-free classification, and 12-month shelf life when stored as directed. The 500 mL bottle size is suitable for R&D teams, pilot-scale process development, and multi-user core facilities that require a defined reagent pack size for controlled inventory. Procurement teams can compare this item with related products in our cell culture reagents category when building standardized supply lists for cell culture support operations.

• Reduced-serum cell culture workflows where serum carryover or serum-derived background must be limited while maintaining a practical liquid medium format.
• Phenol red-free assay preparation, including imaging, fluorescence, absorbance, and other readouts where a pH indicator dye may affect signal interpretation.
• Transient reagent exposure, wash, recovery, or complex-formation steps in cell culture protocols that specify a reduced serum, buffered, animal-derived component-free medium.
• Process development studies requiring documented pH, osmolality, endotoxin, shelf-life, and storage parameters for batch record alignment.
• Academic, biotech, and biopharma R&D settings where animal-origin-free inputs are preferred for material traceability and platform consistency.

Store CCM-RG-0015 at 2–8 °C and protect from light. Do not use the product beyond the stated 12-month shelf life, and minimize repeated warming cycles by aliquoting under validated aseptic conditions if your internal procedure allows. Inspect the bottle before use for container integrity, clarity, and expiration status. The product ships on blue ice to maintain refrigerated transit conditions; upon receipt, transfer immediately to controlled refrigerated storage. For quotation support, temperature-sensitive routing, or scheduled replenishment, use the request quote workflow and include destination, required delivery window, and annual usage estimate.

CellCultureMedia supplies CCM-RG-0015 for research, development, and bioprocessing support under ISO-aligned quality practices appropriate for cell culture media and reagents. Lot-specific documentation, including Certificate of Analysis availability, can be requested for purchasing review, incoming QC, and audit file completion. Documented specifications include pH, osmolality, endotoxin limit, shelf life, storage conditions, formulation attributes, and shipping condition. Additional quality information can be reviewed through our quality resources. Free worldwide shipping is available for standard eligible orders, while bulk, scheduled, or temperature-sensitive shipments can be quoted to match site receiving requirements.