MEM α Medium with Phenol Red and L-Glutamine

CCM-CM-0025 is a 500 mL liquid MEM α formulation for routine research culture, assay development, and upstream process development studies. The medium contains phenol red for visual pH monitoring, 2 mM L-glutamine as a nitrogen source, Earle’s balanced salts for ionic balance, MEM non-essential amino acids, and 1.0 g/L D-glucose. It is manufactured as an animal-origin-free medium before any user-added supplements; where required by a protocol, users commonly supplement with 10% FBS or other qualified additions.

The formulation is suitable for laboratories standardizing work in classical cell culture formulations while maintaining procurement visibility on physical and release specifications. Key release targets include pH 7.0-7.4, osmolality 280-330 mOsmol/kg, endotoxin ≤1 EU/mL, and sterile-filtered liquid presentation. Recommended incubation conditions are 37°C with 5-10% CO2, subject to the requirements of the selected cell type, vessel format, and supplementation strategy. The product is positioned for scientific and basic research use, including cell expansion and experimental culture maintenance where MEM α chemistry is specified by the protocol.

• Expansion and maintenance of fibroblast cultures when MEM α with non-essential amino acids, L-glutamine, and phenol red is specified.
• Culture of primary rat astrocytes and related adherent cell models under 37°C, 5-10% CO2 conditions, with protocol-defined supplementation.
• Comparative media screening, lot qualification studies, and culture condition optimization in R&D laboratories.
• Academic, biotech, and biopharma research workflows requiring sterile-filtered liquid medium with documented pH, osmolality, and endotoxin limits.
• Small-scale process development studies using a consistent 500 mL bottle format for controlled dispensing and inventory planning.

Store unopened bottles at 2-8°C and protect from light to maintain phenol red performance and overall formulation stability. The stated shelf life is 12 months when stored under recommended conditions and within the labeled expiry period. Shipments are packed on blue ice to help maintain refrigerated transit conditions. On receipt, inspect the bottle, cap, fill volume, and appearance before acceptance into controlled storage. Use aseptic technique during dispensing, minimize repeated warming cycles, and return the bottle to 2-8°C promptly after use. Do not use if turbidity, precipitate, leakage, or unexpected color change is observed.

CellCultureMedia supplies CCM-CM-0025 through ISO-aligned manufacturing and quality release workflows appropriate for research and bioprocessing supply chains. Release documentation can include lot-specific Certificate of Analysis information on request, covering applicable attributes such as sterility status, endotoxin specification, pH range, osmolality range, shelf life, and storage requirements. For supplier qualification, documentation routing, or audit support, procurement and QA teams may review our quality information or request batch documentation during ordering.

Free worldwide shipping is available for this 500 mL format, with blue-ice logistics used for temperature-sensitive handling. For multi-bottle demand, recurring supply, institutional purchasing, or scale-up planning, use the request a quote channel to confirm availability, volume pricing, and delivery scheduling.