MEM Alpha Medium with Phenol Red and L-Glutamine

CCM-CM-0026 is a ready-to-use MEM alpha liquid medium designed for adherent cell culture systems that require phenol red and L-glutamine but do not require added nucleosides or deoxynucleosides in the base formulation. It belongs to the classical media portfolio and is intended for research and bioprocess development settings where lot traceability, defined release specifications, and consistent ordering are important. The medium is commonly paired with serum supplementation; the source specification indicates use with 10% fetal bovine serum when required by the cell system.

The formulation includes 1.0 g/L D-glucose, 2 mM L-glutamine, and phenol red. It is supplied in liquid form with a release pH range of 7.0–7.4 and osmolality of 280–330 mOsmol/kg. Sterility is achieved by filtration, and endotoxin is specified at ≤1 EU/mL. The product is classified as animal-origin-free for the base medium; users should evaluate any supplements added downstream against their own sourcing and process requirements. The stated shelf life is 12 months when stored under specified conditions.

• Routine culture of keratinocytes and compatible human pigment-cell research lines when supported by an appropriate serum or supplement strategy.
• Comparative evaluation of MEM alpha-based systems where nucleosides and deoxynucleosides are intentionally excluded from the basal formulation design.
• R&D-scale expansion, assay preparation, and media optimization studies requiring phenol red as a visual indicator of pH drift.
• Laboratory and pilot-scale cell culture workflows operated at 37°C with 5–10% CO2, according to the validated protocol for the relevant cell type.
• Procurement programs that need a 500 mL liquid medium with defined pH, osmolality, sterility, endotoxin, storage, and shelf-life specifications.

Store CCM-CM-0026 at 2–8°C protected from light. Do not expose the bottle to repeated warming cycles, extended room-temperature hold times, or conditions that may compromise L-glutamine stability. The product ships on blue ice to maintain a refrigerated logistics profile during transit. Before use, equilibrate only the required volume to the working temperature and handle aseptically in a qualified biosafety cabinet or equivalent controlled environment. Inspect the bottle for clarity, container integrity, and expected phenol red color before supplementation or use. Once opened, follow internal laboratory hold-time rules and minimize repeated bottle entries.

CellCultureMedia supplies CCM-CM-0026 for research, academic, and bioprocess development purchasing programs that require specification-grade documentation. Manufacturing and quality release are managed using ISO-aligned practices, with filtration-based sterility control and batch-specific testing for key parameters such as pH, osmolality, endotoxin, appearance, and sterility. A Certificate of Analysis is available on request through our quality documentation channel, and procurement teams may request a quote for bulk orders, scheduled supply, or multi-site sourcing. The listed pack size is 500 mL, with price shown from $48.61, and eligible orders include free worldwide shipping subject to route and temperature-control feasibility.