MEM Alpha Medium with Nucleosides, No Phenol Red

CCM-RG-0016 is a liquid MEM Alpha medium configured with L-glutamine, nucleosides, and deoxynucleosides, while excluding phenol red. The product is supplied in a 500 mL format at USD 97.22 for routine laboratory ordering, method development, and scale-up screening. Its animal origin-free classification supports procurement programs that require reduced animal-derived component exposure. Key lot specifications include pH 7.0–7.4, osmolality 280–330 mOsmol/kg, endotoxin ≤1 EU/mL, liquid form, and a 12-month shelf life when stored as directed.

The formulation is suitable when a nutrient-rich MEM Alpha base is required but visual pH indicator dye is not desired. The included nucleoside and deoxynucleoside package can support nucleotide metabolism during growth, recovery, or adaptation studies, while L-glutamine provides a readily available nitrogen and carbon source for many mammalian culture systems. Because phenol red is omitted, the medium is well matched to absorbance, fluorescence, imaging, and color-sensitive analytical workflows. For related buffers, supplements, and media components, see our cell culture reagents category.

• Expansion and maintenance studies for mammalian cells that are compatible with MEM Alpha-type formulations.
• Phenol red-free assay systems where background absorbance, fluorescence interference, or image analysis bias must be minimized.
• Process development experiments comparing media chemistry, osmolality response, nutrient utilization, and passage-to-passage consistency.
• Culture workflows that require L-glutamine already present in the bottle, reducing separate supplementation steps at setup.
• Research use in academic, biotech, and bioprocess laboratories evaluating animal origin-free media inputs for defined supply chains.
• Small-batch qualification before larger-volume purchasing; procurement teams can request quotations and pack planning through request quote.

Store unopened bottles at 2–8 °C and protect from light. The source storage condition specifies refrigerated storage away from light, and shipment is performed on blue ice to maintain a cold-chain profile in transit. Do not freeze unless your internal qualification confirms acceptability for the specific workflow. Before use, inspect the liquid for container integrity, clarity, and unusual color shift, then warm only the required volume using controlled laboratory practice. Handle aseptically after opening, minimize repeated temperature cycling, and record opening date, lot number, and operator for traceability. Use within the stated 12-month shelf life and any shorter in-use period defined by your site procedure.

CellCultureMedia supplies CCM-RG-0016 under ISO-aligned quality expectations for research and bioprocessing customers that need documented, repeatable materials. Lot documentation can be requested to support incoming inspection, including Certificate of Analysis availability, pH, osmolality, endotoxin limit, shelf-life assignment, and other release information where applicable. For procurement files, supplier qualification, and audit preparation, consult our quality documentation resources or request lot-specific records with your order. Free worldwide shipping is available where import rules and cold-chain logistics allow, and our team can coordinate documentation needs for multi-site ordering, standing replenishment, and bulk purchasing programs.