MCDB 153 Medium with Phenol Red and HEPES

CCM-CM-0036 is an MCDB 153 liquid medium supplied in a 500 mL format for human keratinocyte culture work under controlled incubator conditions. The supplied formulation contains phenol red for visual pH monitoring, 1.1 g/L D-glucose as the carbohydrate source, 6 mM L-glutamine, 55 mg/L sodium pyruvate, and HEPES buffering support. The medium is specified for use at 37 ℃ in a 5-10% CO2 atmosphere and is commonly evaluated with 10% FBS as part of a laboratory-defined complete culture system. The liquid is sterile-filtered and intended for scientific and basic research use.

Key release specifications include pH 7.0-7.4, osmolality 305-338 mOsmol/kg, endotoxin ≤1 EU/mL, and a 12-month shelf life when stored as directed. The medium is classified as animal origin-free in the supplied specification; if users add FBS or other supplements, the final prepared system should be qualified against the requirements of the receiving protocol. For related products, procurement teams can review the classical media category or request matched media, sera, and reagent sourcing support through CellCultureMedia.

• Human keratinocyte culture research where MCDB 153 chemistry, controlled osmolality, and buffered liquid format are required.
• Routine expansion, maintenance, and experimental preparation of keratinocyte cultures in academic, biotech, and bioprocess development laboratories.
• Comparative media screening, supplement evaluation, and lot-to-lot qualification studies using phenol red as a visible pH indicator.
• Assay support workflows that require documented sterility, low endotoxin specification, and defined pH range before use.
• Procurement programs consolidating 500 mL liquid media supply with transparent pricing, temperature-controlled shipment, and documentation on request.

Store CCM-CM-0036 at 2-8 ℃ protected from light, and keep the bottle sealed until use. Do not use material beyond the assigned expiration date or if turbidity, particulate contamination, leakage, or abnormal color is observed. Handle aseptically in a qualified biosafety cabinet, minimize repeated warming cycles, and pre-warm only the volume required for the day’s work. Shipping is specified on blue ice to support refrigerated transit; upon receipt, inspect the packout, record the delivery condition for internal traceability, and transfer the bottle to refrigerated storage without delay.

CellCultureMedia supplies CCM-CM-0036 for institutional research and bioprocess support purchasing, with quality controls aligned to ISO-style manufacturing and release documentation practices. Lot-specific Certificate of Analysis documentation can be requested for pH, osmolality, endotoxin, sterility, shelf-life, and other available release attributes. For audit files, incoming inspection, or vendor qualification, buyers may consult our quality documentation resources. The listed 500 mL price is $145.83, with free worldwide shipping offered where routing and cold-chain service levels are available. For standing orders, multi-bottle demand, or project-based forecasts, use request a quote to confirm availability, lead time, and volume pricing.