MCDB 131 Medium with Phenol Red and Sodium Pyruvate

CCM-RG-0017 is a liquid MCDB 131 medium configuration for laboratories that require a defined base for endothelial cell culture workflows. The medium is specified for human microvascular endothelial cells and is commonly paired with 10% fetal bovine serum and any required lab-selected supplements. It includes phenol red for visual pH indication, 1.0 g/L D-glucose as the carbohydrate source, and 110 mg/L sodium pyruvate as an additional carbon and energy substrate. L-glutamine and HEPES are intentionally excluded, giving process engineers and R&D users control over labile glutamine supplementation and buffering strategy.

The product is manufactured as an animal origin-free reagent and supplied sterile-filtered in a 500 mL liquid format. Key release specifications include pH 7.2–7.7, osmolality 260–320 mOsmol/kg, endotoxin ≤1 EU/mL, and a 12-month shelf life when stored as directed. The medium is intended for scientific and basic research applications in controlled cell culture environments. For related formulations and procurement planning, visit the cell culture reagents category.

• Routine research culture of human microvascular endothelial cells under humidified incubator conditions at 37°C and 5–10% CO2.
• Endothelial cell expansion workflows where users add 10% FBS and select additional supplements according to internal protocols.
• Comparative medium evaluation studies requiring MCDB 131 chemistry with phenol red and sodium pyruvate but without L-glutamine or HEPES.
• R&D process development where glutamine stability, buffer selection, pH response, and serum supplementation are managed separately by the laboratory.
• Basic research applications requiring a sterile-filtered, animal origin-free liquid medium with documented pH, osmolality, endotoxin, and shelf-life parameters.

Store CCM-RG-0017 at 2–8°C protected from light. Do not expose the bottle to repeated warming cycles, direct illumination, or non-aseptic handling. The product ships on blue ice to maintain refrigerated transit conditions; upon receipt, inspect the bottle and packaging, record the delivery condition, and transfer the medium promptly to qualified refrigerated storage. Mix gently before use without foaming. Because this configuration does not contain L-glutamine or HEPES, add those components only if required by the validated culture protocol. Use aseptic technique when withdrawing aliquots and avoid returning unused medium to the original container.

CellCultureMedia supplies CCM-RG-0017 for research and bioprocessing organizations that need consistent reagent specifications, responsive quotation support, and international delivery. Manufacturing and quality controls follow ISO-aligned practices, and lot-specific documentation can be requested for procurement files, incoming QC review, or method transfer records. A Certificate of Analysis is available on request and may include applicable release data such as appearance, sterility status, pH, osmolality, endotoxin, shelf life, and storage conditions. For documentation expectations, see our quality information. For volume requirements, scheduled supply, or institutional purchasing, submit details through request a quote; free worldwide shipping is available for qualified orders.