McCoy's 5A Medium with Phenol Red

CCM-CM-0032 is a ready-to-use liquid McCoy's 5A medium configured for laboratories that require a phenol-red-containing formulation with 3.0 g/L D-glucose, 1.5 mM L-glutamine, and plant-derived peptone. The formulation does not include sodium pyruvate or HEPES, allowing process teams to manage carbon source balance, buffering strategy, and supplementation separately. It is categorized within classical media and is suitable for procurement programs standardizing commonly used media across R&D, cell biology, and upstream development groups.

The medium is specified for mammalian cell culture including rat fibroblast culture and similar adherent cell workflows. As supplied, it is an animal-origin-free base formulation; routine use may require external serum supplementation, commonly 10% fetal bovine serum depending on the cell model and internal protocol. Culture conditions are typically 37 °C with 5-10% CO2. Key release specifications include filtered sterility, endotoxin not more than 1 EU/mL, pH 6.6-7.1, osmolality 280-330 mOsmol/kg, and 12-month shelf life when stored as directed.

• Routine propagation and maintenance of rat fibroblasts and comparable mammalian adherent cell lines under CO2 incubator conditions.
• Research-scale culture workflows requiring McCoy's 5A chemistry with phenol red for visual pH indication during routine handling.
• Experimental designs where sodium pyruvate and HEPES are intentionally excluded so laboratories can add them separately if needed.
• Serum-supplemented culture systems, including protocols calling for approximately 10% fetal bovine serum after laboratory qualification.
• Process development and method comparison studies where pH, osmolality, endotoxin, and sterility specifications must be documented for lot review.

Store unopened bottles at 2-8 °C and protect from light to maintain phenol red performance and formulation integrity through the stated 12-month shelf life. The product ships on blue ice; on receipt, inspect packaging, confirm the product remains cool, and transfer promptly to refrigerated storage. Do not freeze unless validated by your laboratory, and avoid repeated warm exposure during aliquoting. Use aseptic technique after opening, minimize time at room temperature, and record lot number, opening date, and expiry date in the laboratory inventory system.

CellCultureMedia supplies CCM-CM-0032 for research and bioprocessing use with specification-driven lot control. Manufacturing and release practices are ISO-aligned, and lot documentation can be reviewed through our quality program. A Certificate of Analysis is available on request and can support incoming inspection, vendor qualification, and batch record attachment. Current package size is 500 mL at $97.22, with free worldwide shipping where service is available. For volume requirements, scheduled replenishment, or multi-site supply planning, submit a request for quote.