M2 Medium with Phenol Red, HEPES and Lactate

M2 medium is commonly used as a handling and manipulation medium in reproductive biology, embryo research models, gamete handling workflows, and related cell culture procedures where short-term environmental stability is important. This formulation contains sodium lactate as a key energy substrate, sodium bicarbonate for carbonate buffering, HEPES for additional buffering capacity under ambient handling conditions, and phenol red for visual pH monitoring. It is supplied as a ready-to-use liquid in a 50 mL format for laboratories that require small-volume, specification-controlled reagent lots.

CCM-RG-0095 is classified as an animal-origin formulation and is provided without penicillin and streptomycin, giving process teams direct control over antimicrobial-use policies in their protocols. Release specifications include pH 7.2 to 7.6 and osmolality 280 to 304 mOsmol/kg, supporting consistency for sensitive cell handling steps. The endotoxin specification is ≤10 EU/mL, and the product has a stated shelf life of 12 months when stored under recommended conditions. For related buffers, media, and supplements, see the cell culture reagents category.

• Short-term handling of cells and biological samples in research environments requiring a balanced M2 medium with bicarbonate and HEPES buffering.
• Workflows where sodium lactate is required as part of the medium chemistry and where phenol red pH indication is useful during bench handling.
• Research protocols that specify an M2 medium without penicillin and streptomycin, including processes where antibiotic-free conditions are part of experimental design.
• Procurement programs that require defined specifications for pH, osmolality, endotoxin, shelf life, and refrigerated storage conditions before purchase approval.
• Small-batch R&D, academic core facility use, assay development, and method-transfer activities where a 50 mL liquid reagent pack reduces waste and simplifies inventory control.

Store CCM-RG-0095 at 2 to 8 °C protected from light. Do not leave the bottle at room temperature longer than necessary during use, and return unused material promptly to refrigerated storage after aseptic withdrawal. The product ships on blue ice to maintain a refrigerated transit profile. Users should inspect the container on receipt, confirm labeling and expiry, and avoid use if the liquid shows unexpected turbidity, discoloration beyond normal phenol red variation, leakage, or compromised packaging. For best inventory practice, record lot number, receipt date, first-open date, and remaining volume in the laboratory reagent log.

CellCultureMedia supplies CCM-RG-0095 for research and bioprocessing use with lot-level traceability and specification-oriented documentation. Manufacturing and release practices are managed under ISO-aligned quality principles, with attention to formulation consistency, controlled filling, refrigerated logistics, and documented release checks. A Certificate of Analysis can be requested for lot review, including available release specifications such as pH, osmolality, endotoxin limit, appearance, shelf life, and storage conditions. For documentation expectations, visit quality and documentation, or use request a quote for institutional pricing, standing orders, or volume supply. Free worldwide shipping is available for eligible orders and quoted shipments.