M199 Medium, Phenol Red, L-Glutamine, HEPES-Free

M199 medium is a classical liquid culture medium used where a balanced salt, amino acid, vitamin, glucose, and indicator system is required for routine in vitro cell maintenance and experimental culture. CCM-CM-0033 is supplied as a ready-to-use 500 mL liquid formulation containing phenol red for visual pH monitoring, 1.0 g/L D-glucose as the carbohydrate source, and 100 mg/L L-glutamine as a nitrogen and energy-related nutrient. The formulation does not contain HEPES, making it suitable for users who manage buffering through bicarbonate and controlled CO2 incubation.

This product is specified for culture at 37 degrees C in 5-10% CO2 and is commonly paired with 10% fetal bovine serum when serum supplementation is required by the cell system. Key release specifications include pH 7.1-7.4, osmolality 270-310 mOsmol/kg, endotoxin not more than 1 EU/mL, and filter-sterilized liquid format. For related formulations, procurement teams can review our classical media portfolio and consolidate sourcing through CellCultureMedia.

• Culture support for chicken embryo fibroblasts and similar primary or established adherent cell models used in scientific and basic research.
• Routine cell expansion, maintenance, and experimental setup where M199 chemistry is specified by an internal protocol or published method.
• CO2-incubator workflows requiring a medium without HEPES, allowing process engineers to control buffering through bicarbonate equilibrium and incubator settings.
• Serum-supplemented culture systems, typically with 10% fetal bovine serum added aseptically by the end user before use.
• Comparability studies, media screening, and reagent standardization where documented pH, osmolality, endotoxin, sterility, and shelf-life values are needed for purchasing records.

Store CCM-CM-0033 at 2-8 degrees C protected from light. The product is shipped on blue ice and should be transferred to refrigerated storage promptly after receipt. Do not use if the container integrity is compromised, if turbidity or unexpected color change is observed, or if the medium has exceeded its assigned shelf life. The stated shelf life is 12 months when stored unopened under recommended conditions. Warm only the required volume before use, and handle aseptically after opening to maintain performance in cell culture workflows.

CCM-CM-0033 is manufactured under ISO-aligned quality practices for research and bioprocessing supply chains, with filter sterilization and lot-based specification review. A Certificate of Analysis is available on request and may include pH, osmolality, endotoxin, sterility, shelf-life, and formulation confirmation. See our quality documentation information for procurement support. For standing orders, institutional purchasing, or bulk requirements, use the request a quote form; the listed 500 mL unit price is USD 48.61 and free worldwide shipping is available.