Low-Glucose DMEM with Phenol Red, Glutamine and Pyruvate
Low-glucose DMEM with phenol red, L-glutamine, and sodium pyruvate is a liquid classical cell culture medium formulated for routine growth and maintenance of adherent mammalian cells. CCM-CM-0011 is supplied as a 500 mL, animal-origin-free formulation containing 1.0 g/L D-glucose and no HEPES. It is suited to fibroblasts, smooth muscle cells, and comparable cultures run at 37 °C with 5–10% CO2. Free worldwide shipping is available, and related formulations can be reviewed under classical media.
- ✓ Free worldwide shipping
- ✓ ISO-aligned manufacturing
- ✓ Certificate of Analysis on request
- ✓ Bulk & custom volumes quoted on request
Overview
CCM-CM-0011 is a low-glucose Dulbecco's Modified Eagle Medium for cell culture workflows requiring a defined classical medium with phenol red as a visual pH indicator. The formulation includes 1.0 g/L D-glucose, 4 mM L-glutamine, and 0.11 g/L sodium pyruvate, and it is manufactured without HEPES. The reduced glucose level is commonly selected for cell types and experimental designs where high-glucose conditions are not desired. Sodium pyruvate provides an additional carbon and energy substrate, while L-glutamine supports nitrogen metabolism in standard mammalian culture systems.
This liquid medium is intended for scientific and basic research use in laboratories, core facilities, and process-development groups. Typical use requires supplementation with 10% fetal bovine serum or an equivalent validated supplement according to the user's cell line protocol. Key release specifications include pH 7.0–7.4, osmolality 310–340 mOsmol/kg, endotoxin ≤1 EU/mL, and filtration-based sterility processing. Procurement teams can use these values for incoming material checks, lot comparison, and documentation alignment across multi-site cell culture operations.
Applications
- Routine culture of fibroblasts, smooth muscle cells, and other adherent mammalian cells compatible with low-glucose DMEM chemistry.
- Research-scale expansion, maintenance, and assay preparation where phenol red is useful for visual monitoring of medium color shift during incubation.
- CO2 incubator culture at 37 °C with 5–10% CO2, using validated serum supplementation, commonly 10% fetal bovine serum.
- Comparative formulation studies where a HEPES-free medium is required to control buffering variables under bicarbonate and CO2 conditions.
- Academic, biotechnology, and biopharma R&D workflows requiring a ready-to-use, filter-sterilized classical medium with documented pH, osmolality, and endotoxin limits.
Storage and Handling
Store CCM-CM-0011 at 2–8 °C protected from light. The product is shipped on blue ice to help maintain refrigerated conditions during transit, with free worldwide shipping available for eligible orders. Do not freeze unless the receiving laboratory has validated freeze-thaw handling for this specific formulation. Use aseptic technique after opening, minimize repeated warming cycles, and record bottle opening dates in accordance with internal quality procedures. The stated shelf life is 12 months when unopened and stored under the recommended conditions. Because the medium contains L-glutamine and phenol red, prolonged exposure to light, elevated temperature, or non-sterile handling may affect performance characteristics.
Quality and Documentation
CellCultureMedia supplies CCM-CM-0011 for research and bioprocessing environments that require traceable, specification-driven cell culture materials. Manufacturing and release practices are ISO-aligned, and lot documentation can be provided to support receiving inspection, audit files, and change-control review. A Certificate of Analysis is available on request and may include release data such as appearance, pH, osmolality, sterility status, endotoxin level, and shelf-life information. For procurement planning, request a quotation through request quote with expected volume, delivery location, and recurring demand. Additional information about documentation practices is available on the quality page.
Specifications
Formulation Highlights
Contains (+)
Phenol red, 1.0 g/L D-glucose, 4 mM L-glutamine, 0.11 g/L sodium pyruvate
Does Not Contain (-)
[-]HEPES
Storage & Handling
Storage: 2~8 ℃, protected from light. Shelf life: 12 months. Ship cold-chain where required.
Quality & Documentation
Manufactured under ISO-aligned quality management systems. Full documentation — Certificate of Analysis (CoA), Material Safety Data Sheet (MSDS), formulation summary, and stability data — is available on request. Email [email protected] with the SKU.
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- DMEM (Dulbecco's Modified Eagle Medium)DMEM (Dulbecco's Modified Eagle Medium) is a basal cell culture medium derived from Eagle's Minimal Essential Medium by raising the amino acid and vitamin concentrations roughly fourfold. It is supplied in high-glucose (4,500 mg/L, 25 mM) and low-glucose (1,000 mg/L, 5.6 mM) forms, buffered with 3,700 mg/L sodium bicarbonate, and requires serum or a defined supplement plus a CO2 atmosphere to hold physiological pH. DMEM is the default medium for adherent lines such as HEK293, HeLa, NIH/3T3, Vero and CHO-derived adherent cultures, and for most primary fibroblasts.
- Glucose solution in cell cultureA cell culture glucose solution is a concentrated sterile D-glucose stock, commonly supplied at 300-450 g/L (30-45% w/v), used to supplement basal media and to feed cultures that consume glucose faster than the medium supplies it. D-glucose has a molecular weight of 180.16, so 1 g/L equals 5.55 mM: standard media run from 1 g/L (5.5 mM) in low-glucose DMEM through 2 g/L (11.1 mM) in RPMI 1640 to 4.5 g/L (25 mM) in high-glucose DMEM. Glucose is added to prevent depletion in long or high-density cultures, and it is normally sterile-filtered rather than autoclaved, because heating glucose with amino acids produces browning reaction products.
- EMEM vs DMEM: What the Modification Actually ChangedEMEM (Eagle's Minimum Essential Medium, also sold as MEM) and DMEM (Dulbecco's Modified Eagle Medium) are the same medium one generation apart: DMEM is Eagle's formulation enriched, with roughly four times the vitamins, about twice most amino acids, twice the glutamine, added glycine and serine, ferric nitrate, and 3.7 g/L sodium bicarbonate against EMEM's 1.5-2.2 g/L. The practical consequence is that DMEM supports fast-growing, metabolically demanding lines such as HEK293 and NIH/3T3, while EMEM suits slower, less demanding adherent cells and primary lines - and because DMEM's higher bicarbonate is formulated for 10% CO2 while EMEM's suits 5%, the two are not interchangeable without checking your incubator.
