Leibovitz’s L-15 Medium with Phenol Red

Leibovitz’s L-15 medium is a classical liquid cell culture medium used where defined inorganic salts, amino acids, energy substrates, and pH indication are required for routine cell maintenance and experimental culture work. CCM-CM-0031 is supplied as a ready-to-use 500 mL liquid medium with phenol red for visual pH monitoring, 0.9 g/L D-galactose as the listed carbohydrate source, 300 mg/L L-glutamine, and 0.55 g/L sodium pyruvate. The formulation is specified without D-glucose, which makes it suitable for laboratories that require an L-15 chemistry built around galactose and pyruvate rather than glucose.

The product is categorized under classical media and is manufactured for research and process-development environments that require consistent procurement specifications. Source specifications include liquid form, filtered sterility, endotoxin ≤1 EU/mL, pH 7.1–7.8, and osmolality of 280–330 mOsmol/kg. The medium is described as animal-origin-free as supplied; however, the recommended complete-medium workflow requires supplementation with 10% fetal bovine serum or an equivalent lab-validated supplement. Incubation guidance from the source data is 37 °C with 5–10% CO2; users should confirm final gas conditions against their cell-line SOP, vessel format, and serum or supplement system.

• Routine culture of monkey kidney cells and comparable mammalian cell types requiring Leibovitz’s L-15 chemistry.
• Research workflows where a D-glucose-free medium containing D-galactose, L-glutamine, and sodium pyruvate is specified.
• Serum-supplemented culture protocols using 10% fetal bovine serum or a qualified alternative supplement after in-house suitability testing.
• Assay development, media comparison studies, and cell maintenance programs that require documented pH, osmolality, sterility, and endotoxin limits.
• Procurement for academic, biotech, and bioprocess development laboratories standardizing on 500 mL liquid medium bottles for controlled inventory turnover.

For price confirmation, lot availability, or consolidated purchasing across multiple media and reagents, buyers can submit requirements through the request quote page. Include CCM-CM-0031, desired quantity, delivery location, and any documentation requirements so the commercial team can align quotation, shipping, and batch reservation details.

Store CCM-CM-0031 at 2–8 °C protected from light. Do not freeze unless validated internally for the specific workflow, because freezing can alter salt balance, pH appearance, or component solubility after thawing. The stated shelf life is 12 months when unopened and stored under the specified conditions. Handle using aseptic technique after opening, minimize repeated warming, and return the bottle to refrigerated storage promptly after use. The product ships on blue ice to maintain a refrigerated transport profile. Inspect bottles on receipt for leakage, clarity, labeling, and temperature-control evidence before release into laboratory inventory.

CellCultureMedia supplies CCM-CM-0031 for research, cell culture, and bioprocess-development use with specification-focused documentation for purchasing and QA review. Manufacturing and release controls are ISO-aligned, and lot-specific documentation such as a Certificate of Analysis can be requested for sterility, endotoxin, pH, osmolality, shelf-life, and identification details where available. For quality-system review, visit the quality page or request the required documentation during quotation. The product is offered at $97.22 per 500 mL bottle, with free worldwide shipping available for eligible orders and consolidated purchasing support for multi-item media programs.