Hybridoma Serum-Free Feed Supplement Kit

This hybridoma feed supplement kit is a concentrated liquid input for serum-free production processes using hybridoma cells. It is intended to replenish nutrients and support extended culture duration where a feed strategy is preferred over simple batch culture. The product is animal-origin-free and supplied as a sterile-filtered liquid, helping process engineers reduce raw-material complexity and simplify documentation for research and bioprocessing programs.

Key specifications include pH 10.0 to 10.6, osmolality 900 to 1100 mOsmol/kg, endotoxin not more than 20 EU/mL, and 12-month shelf life when stored as directed. The recommended culture environment for the target cell type is 37°C with 5 to 10% CO2. Because this is a feed supplement rather than a complete growth environment, feed timing, addition volume, and base medium compatibility should be verified at small scale before routine use. Related serum-free formats can be reviewed in our serum-free media category.

• Feeding of hybridoma cells in serum-free culture where serum addition is not required.
• Process development studies comparing feed concentration, timing, and cumulative addition strategy.
• Small-scale screening in flasks, deep-well plates, or spinner formats before scale-up.
• Biopharma and vaccine-manufacturing research workflows requiring defined, animal-origin-free inputs.
• Antibody expression process support in controlled laboratory and pilot-scale culture systems.
• Raw-material standardization programs where documented pH, osmolality, sterility, endotoxin, shelf life, and storage conditions are needed for supplier files.

Store CCM-SF-0032 at 2 to 8°C protected from light. Do not leave the bottle at room temperature longer than required for aseptic use, and mix gently before sampling if the process protocol requires homogenization. The product is shipped on blue ice to maintain refrigerated transit conditions. Use aseptic technique after opening, minimize repeated temperature excursions, and record open-bottle handling according to your site procedure. The stated shelf life is 12 months under unopened, recommended storage conditions.

CellCultureMedia supplies CCM-SF-0032 for professional cell culture, R&D, and bioprocessing procurement programs. Manufacturing and release are managed under ISO-aligned quality practices, with sterile filtration and lot-level specifications for endotoxin, pH, osmolality, appearance, and shelf life where applicable. A Certificate of Analysis is available on request through our quality documentation team. For price locks, scheduled deliveries, or multi-unit requirements, submit details through request a quote; free worldwide shipping is available for this item.