High-Glucose DMEM for PSC/ESC, No L-Glutamine

High-glucose DMEM is a widely used nutrient platform for pluripotent stem cell and embryonic stem cell culture systems that require controlled carbohydrate availability and a defined starting medium. CCM-SC-0006 is supplied without L-glutamine, allowing process engineers and R&D teams to add fresh L-glutamine or an alternative glutamine source at the point of use according to internal SOPs. The medium includes D-glucose and phenol red for routine culture monitoring, with phenol red providing a visual indication of pH drift during incubator handling and media changes.

This 500 mL format is suitable for research-scale culture, qualification runs, and small-batch procurement where lot traceability is important. The supplied medium is specified as animal-origin-free before customer-added supplements; protocols calling for serum should add 10% fetal bovine serum or an internally qualified equivalent. Key release specifications include filter sterilization, endotoxin not more than 1 EU/mL, pH 7.1-7.6, osmolality 265-290 mOsmol/kg, and a 12-month shelf life when stored as directed. Related options are available in our stem cell media category for labs standardizing PSC/ESC workflows.

• PSC and ESC maintenance workflows requiring a high-glucose DMEM nutrient environment with user-controlled glutamine addition.
• Serum-containing culture systems where the working medium is completed with 10% fetal bovine serum immediately before use or according to site-specific procedure.
• Protocol transfer, media comparison, and incoming-material qualification for academic, biotechnology, and bioprocess development laboratories.
• Incubator-based culture at 37 °C and 4-6% CO2, subject to cell-line-specific validation by the user.
• Research and basic science applications where a filter-sterilized, phenol-red-containing DMEM is required for reproducible handling.

Store CCM-SC-0006 at 2-8 °C protected from light. Do not freeze unless the customer has validated freezing for the complete formulation and intended application. The product is shipped on blue ice to support refrigerated transport conditions; inspect bottles on receipt for intact packaging, clarity, and correct labeling before acceptance into inventory. Use aseptic technique when opening, and minimize repeated warming cycles by aliquoting under controlled conditions if permitted by local SOPs. Because the formulation does not contain L-glutamine, add the selected glutamine source only after the medium has reached the required handling temperature and immediately before use where stability is a concern.

CellCultureMedia supplies CCM-SC-0006 for research and bioprocess development environments that require documented specifications, predictable logistics, and procurement-ready packaging. Manufacturing and release controls are ISO-aligned, with sterility, endotoxin, pH, osmolality, appearance, labeling, and shelf-life attributes managed through controlled quality systems. A Certificate of Analysis can be requested for lot review, vendor qualification, or material intake records through our quality documentation channel. For multi-bottle orders, recurring demand, or institution-wide purchasing, procurement teams can request a quote for volume pricing, lead-time confirmation, and shipping coordination. Free worldwide shipping is available for this product, including blue-ice shipment where required for temperature-sensitive transit.