Glucose-Free RPMI 1640 Medium

CCM-CM-0016 is a glucose-free RPMI 1640 liquid medium for laboratories that need to remove D-glucose from the culture environment while retaining key RPMI 1640 characteristics. The formulation includes phenol red for visual pH monitoring and 300 mg/L L-glutamine as a nitrogen source, while excluding both D-glucose and HEPES. It is suitable for scientists comparing carbohydrate restriction, supplement-defined conditions, metabolic response, or media optimization parameters in RPMI-adapted systems.

The product is supplied as a 500 mL sterile liquid medium in the classical media portfolio. Source specifications include animal-origin-free classification, filter sterilization, endotoxin not more than 1 EU/mL, pH 7.0–7.4, and osmolality 260–305 mOsmol/kg. For routine culture use, supplementation with 10% fetal bovine serum is recommended unless a validated alternative supplement strategy is used. Incubation conditions are typically 37 °C in a humidified 5–10% CO2 atmosphere, with final performance depending on cell type, serum lot, seeding density, and the intended experimental design.

• Culture of PBMC and other suspension or semi-suspension cell models that are compatible with RPMI 1640 chemistry and require user-controlled glucose addition.
• Metabolic studies where D-glucose must be absent from the base formulation so that investigators can add defined carbohydrate concentrations independently.
• Serum-supplemented research workflows using 10% fetal bovine serum with RPMI-adapted immune, hematopoietic, or primary-cell-derived research models.
• Comparative media screening in which glucose-free, HEPES-free, phenol-red-containing RPMI 1640 is benchmarked against standard glucose-containing formulations.
• Process development and analytical cell culture experiments where pH indication, L-glutamine presence, low endotoxin specification, and sterile liquid supply are procurement requirements.

Store CCM-CM-0016 at 2–8 °C protected from light. Do not freeze, and avoid repeated warming cycles that may affect pH, glutamine stability, or visual appearance. The stated shelf life is 12 months when the bottle is unopened and maintained under recommended refrigerated, light-protected conditions. Before use, inspect the container for integrity, clarity, particulate matter, and expected color. Warm only the required volume to room temperature or the validated process temperature, and use aseptic technique when adding serum or other supplements. Because the formulation does not contain HEPES, CO2 equilibration should be considered when setting up incubator conditions and when interpreting phenol red color changes.

CellCultureMedia supplies CCM-CM-0016 for research and bioprocess development procurement programs that require consistent specification review, lot traceability, and responsive documentation. Manufacturing and release are managed within ISO-aligned quality practices, with sterility by filtration, endotoxin limit, pH range, osmolality range, storage condition, and shelf-life information available for technical evaluation. A Certificate of Analysis can be requested through our quality documentation team, and procurement groups can submit volume, delivery, or standing-order requirements through request a quote. Standard 500 mL units are listed at $97.22, with free worldwide shipping where available and bulk pricing offered for qualified institutional orders.