Exosome Production Serum-Free Medium

CCM-SF-0070 is a ready-to-use, serum-free medium for exosome production workflows that require defined composition, low background interference, and compatibility with downstream extracellular vesicle isolation. It is intended for mammalian cell culture in research and bioprocess development environments, including both suspension and adherent cell formats. As part of the serum-free media portfolio, this product supports laboratories moving away from serum-containing systems to reduce lot-to-lot variability and animal-derived component exposure.

The medium is supplied as a liquid and is animal-origin-free. It is formulated with D-glucose as a carbon source, L-glutamine as a nitrogen and energy metabolism component, and sodium bicarbonate for CO2-buffered culture systems. Phenol red is excluded to reduce colorant-related analytical interference in extracellular vesicle characterization, imaging-adjacent assays, and downstream sample processing. Recommended culture conditions are 36-38°C with 5-10% CO2, subject to the requirements of the selected mammalian cell type and vessel format. Key release specifications include pH 7.0-7.4, osmolality 280-320 mOsmol/kg, endotoxin not more than 1 EU/mL, and filter sterilization. Shelf life is 12 months when stored as specified.

• Serum-free culture of multiple mammalian suspension cells for extracellular vesicle and exosome collection.
• Serum-free maintenance or conditioning of adherent mammalian cell cultures prior to harvest of conditioned medium.
• Process development studies comparing serum-containing and serum-free extracellular vesicle production conditions.
• Defined-medium workflows where phenol red-free formulation is preferred for downstream particle, protein, nucleic acid, or imaging-related analytical methods.
• Scale-up evaluation in flasks, roller bottles, multilayer vessels, shake flasks, or compatible bioreactor formats after cell-line-specific qualification.
• Academic, biotechnology, and biopharma R&D programs requiring animal-origin-free media for controlled cell culture manufacturing studies.

Store CCM-SF-0070 at 2-8°C protected from light. Do not freeze unless a site-specific stability assessment has been completed, because freeze-thaw exposure may alter medium performance. Before use, mix gently by inversion and warm only the required volume to working temperature; avoid repeated warming of the full bottle. Use aseptic technique after opening, and keep containers tightly closed when not in use. The product is shipped on blue ice to maintain refrigerated transport conditions. For best comparability between experiments, record bottle lot number, opening date, passage number, seeding density, culture duration, CO2 level, and harvest timing.

CellCultureMedia supplies CCM-SF-0070 for organizations that require procurement-ready specifications, lot traceability, and consistent documentation. Manufacturing and release controls are ISO-aligned, with sterility by filtration, endotoxin specification of ≤1 EU/mL, pH 7.0-7.4, osmolality 280-320 mOsmol/kg, and 12-month shelf life under refrigerated, light-protected storage. A Certificate of Analysis can be requested through the quality documentation team, including lot-specific release data when available. For repeat supply, standing orders, or multi-bottle purchasing, submit requirements through request a quote. Free worldwide shipping is available, with refrigerated blue-ice packing arranged according to destination and order profile.