DMEM/F-12 (1:1) Medium with Phenol Red and HEPES

CCM-CM-0017 is a ready-to-use, liquid DMEM/F-12 (1:1) medium designed for laboratories that require a balanced nutrient platform with DMEM amino acid and vitamin content combined with the trace components and nutrient profile of F-12. It is classified as an animal-origin-free supplied formulation and is commonly used as a classical medium for neuronal, glial, endothelial, epithelial, and other mammalian cell culture models. For comparable formulations and procurement planning, see the classical media category.

The medium contains phenol red as a visual pH indicator, 3.15 g/L D-glucose as the carbohydrate source, 2.5 mM L-glutamine for nitrogen and energy metabolism support, and HEPES for added buffering capacity during handling outside the incubator. Source specifications list pH at 7.0-7.4 and osmolality at 290-330 mOsmol/kg. The recommended culture environment is 37℃ with 5-10% CO2. For the indicated workflows, users should supplement with 10% fetal bovine serum or an internally qualified serum alternative according to the cell system and protocol.

• Routine culture of neuronal and glial cell models requiring a DMEM/F-12 nutrient balance and HEPES-assisted pH stability.
• Endothelial cell research workflows where a sterile liquid medium is supplemented by the user with serum, growth factors, or project-specific additives.
• Academic and industrial R&D protocols that require pH 7.0-7.4, osmolality of 290-330 mOsmol/kg, and low endotoxin specification.
• Media screening, cell maintenance, expansion, and experimental comparability studies using a 1:1 DMEM and F-12 formulation.
• Procurement programs needing consistent 500 mL bottle format, documented sterility by filtration, and lot-level quality records.

Store CCM-CM-0017 at 2-8℃ protected from light. Do not freeze unless validated by your internal protocol, as freeze-thaw exposure may alter medium appearance or performance. The product is shipped on blue ice to maintain cold-chain conditions during transit. Before use, inspect the bottle for integrity, color shift, turbidity, precipitate, and expiration date. Warm only the volume required for the session using controlled laboratory practice, and handle aseptically in a biosafety cabinet. The stated shelf life is 12 months when the product is stored unopened under the recommended conditions.

This medium is manufactured under ISO-aligned quality practices and is filter sterilized. Key release specifications include sterility by filtration, endotoxin ≤1 EU/mL, pH 7.0-7.4, and osmolality 290-330 mOsmol/kg. A Certificate of Analysis can be requested for lot verification, incoming QC, and supplier qualification; documentation support is available through our quality team. CellCultureMedia supports R&D groups, process development teams, and purchasing departments with free worldwide shipping where applicable. For multi-bottle requirements, scheduled supply, or project pricing, submit details through request a quote.