CO2 Independent Medium with Phenol Red

CCM-CM-0038 is a liquid CO2 independent medium supplied with phenol red, L-glutamine, and sodium pyruvate, and without HEPES. It is intended for scientific and basic research workflows involving multiple mammalian suspension and adherent cell types. The formulation provides a visible phenol red pH indicator, a ready nitrogen source through L-glutamine, and sodium pyruvate as an additional metabolic substrate. Recommended qualification conditions include 37°C incubation with 5-10% CO2, using 10% FBS supplementation where required by the cell model. For related formulations, see our classical media category.

The supplied medium is classified animal-origin-free before user-added serum or supplements. Key release specifications include liquid form, sterile-filtered manufacture, endotoxin not more than 1 EU/mL, and pH 7.0-7.4. The listed pack size is 500 mL at USD 241.67, supporting small-batch R&D, media screening, and controlled procurement for laboratories standardizing on defined release criteria.

• Routine culture support for many mammalian suspension cells when compatibility has been confirmed by in-house testing.
• Adherent mammalian cell workflows requiring a liquid medium with phenol red for rapid visual pH assessment.
• Short handling intervals, transport between workstations, and bench operations where pH monitoring is important.
• Research-scale process development using 10% FBS supplementation and controlled incubation at 37°C with 5-10% CO2.
• Comparative media evaluation where HEPES-free chemistry is required for study design or process consistency.
• Academic, biotech, and biopharma procurement programs that require sterile-filtered, endotoxin-controlled classical culture media with defined shelf life.

Store CCM-CM-0038 at 2-8°C protected from light. Do not use the medium beyond the assigned 12-month shelf life or after visible changes inconsistent with normal phenol red color response. Shipments are packed on blue ice to support refrigerated transit. On receipt, inspect the bottle and temperature condition, record the lot number, and move the product promptly to refrigerated storage. Use aseptic technique when opening, and avoid repeated warming cycles that can affect cell culture performance. Because the medium contains L-glutamine, laboratories should qualify storage duration after first opening under their internal handling procedures.

CellCultureMedia supplies CCM-CM-0038 under specification-controlled, ISO-aligned manufacturing and release documentation practices. Lot-specific quality attributes include sterility filtration status, pH range, endotoxin limit, shelf life, formulation additives, storage conditions, and shipping conditions. A Certificate of Analysis is available on request through our quality documentation process. Procurement teams needing multiple bottles, scheduled supply, or site-level standardization can submit an RFQ through request a quote. Free worldwide shipping is available, with blue-ice packing used for this refrigerated liquid product.