CHO 18 Serum-Free ADCF Medium with HT Supplement

This CHO 18 serum-free ADCF medium is a ready-to-use liquid formulation for mammalian cell culture processes requiring defined raw materials and reduced animal-origin exposure. The formulation includes D-glucose and HT supplement and is manufactured without phenol red and without L-glutamine, giving process teams greater control over indicator interference, glutamine feeding strategy, and lot-to-lot input definition. It is suitable for users evaluating serum-free media for clone screening, scale-down process models, seed train development, and suspension culture optimization.

Key release specifications include sterile-filtered presentation, pH 7.0 to 7.4, osmolality 270 to 340 mOsmol/kg, and endotoxin not more than 3 EU/mL. The medium is intended for culture at 37 ℃ in 5 to 10% CO2. The animal-origin-free classification supports procurement programs that require traceable, defined components for CHO-based research, vaccine-related process development, and biopharmaceutical manufacturing support activities.

• Serum-free cultivation of CHO K1, suspension-adapted CHO, GS-selected CHO, and CHO DG44 cells.
• Development and optimization of CHO expression workflows where HT supplementation is required.
• Evaluation of phenol-red-free conditions for colorimetric assay compatibility, optical monitoring, or reduced indicator background.
• Processes where L-glutamine is managed separately through timed addition, stable dipeptide alternatives, or feeding studies.
• Research-scale and process-development work supporting recombinant protein production, vaccine-related manufacturing studies, and biopharmaceutical upstream development.
• Procurement-qualified supply for laboratories that require defined serum-free and animal-origin-free liquid media in 500 mL bottles.

Store at 2 to 8 ℃ protected from light. Do not freeze unless an internal validation protocol has confirmed product performance after freezing and thawing. Use aseptic technique after opening, minimize repeated warming, and return unused material to refrigerated storage promptly. The stated shelf life is 12 months when stored unopened under recommended conditions. Product ships on blue ice; inspect containers on arrival, record receiving temperature where required by site procedure, and equilibrate the bottle to the intended working temperature before use.

CellCultureMedia supplies CCM-SF-0008 for research, development, and bioprocessing procurement channels that require documented specifications and consistent logistics. Manufacturing and release documentation follow ISO-aligned quality practices, with sterility, endotoxin, pH, osmolality, appearance, and shelf-life attributes managed through controlled quality systems. A Certificate of Analysis is available on request through our quality documentation workflow. For volume planning, controlled resupply, or multi-site purchasing, submit requirements through request a quote. Free worldwide shipping is available, with cold-chain packing selected according to destination and shipment duration.