Hybridoma Serum-Free Medium, Phenol Red-Free

CCM-SF-0053 is a ready-to-use liquid serum-free medium for hybridoma cells grown under standard humidified incubator conditions of 37℃ and 5–10% CO2. The formulation is chemically defined and animal-origin-free, supporting controlled experimental design and more predictable lot-to-lot performance than serum-containing culture systems. It is phenol red-free for workflows where colorimetric interference, optical background, or assay readout sensitivity must be managed. L-glutamine is not included, allowing users to select the preferred glutamine source, concentration, and feeding strategy for their specific hybridoma platform.

Key release specifications include pH 7.0–7.4, osmolality 270–340 mOsmol/kg, and endotoxin ≤3 EU/mL. The product is supplied as a 500 mL liquid medium and has a stated shelf life of 12 months when stored as directed. D-glucose is included as a core nutrient component. This specification profile makes CCM-SF-0053 suitable for R&D groups and process teams that require a defined medium for hybridoma expansion, antibody expression studies, media screening, or transfer from serum-containing conditions to lower-variability serum-free culture.

• Routine culture and expansion of hybridoma cells in serum-free, animal-origin-free conditions.
• Development and comparison of defined media strategies for monoclonal antibody production research.
• Phenol red-free assay environments where reduced optical or colorimetric background is preferred.
• Process development studies requiring documented pH, osmolality, endotoxin, shelf-life, and storage specifications.
• Adaptation programs moving hybridoma cultures from serum-containing media into defined serum-free formats.
• Small-batch procurement for academic, biotech, and bioprocessing teams requiring 500 mL liquid media with quotation support through CellCultureMedia request-quote services.

Store CCM-SF-0053 at 2–8℃ protected from light. Do not use the product beyond the labeled shelf life or if the container integrity is compromised. Shipments are handled on blue ice to maintain the recommended temperature range during transit; upon receipt, inspect the package, confirm product identity, and transfer the bottle promptly to refrigerated storage. Because L-glutamine is not included, supplement only with qualified materials appropriate to the user’s cell line and process design. For serum-free adaptation, gradual weaning from serum-containing culture may be preferable to direct transfer, depending on the hybridoma line and historical culture conditions.

CellCultureMedia supplies CCM-SF-0053 for research and bioprocessing use with procurement-focused documentation support. Manufacturing and release practices are organized under ISO-aligned quality expectations, with lot-specific Certificate of Analysis availability on request. Typical documentation can support review of product identity, format, storage conditions, pH range, osmolality range, endotoxin limit, shelf life, and shipping conditions. For supplier qualification, technical review, or recurring replenishment planning, visit CellCultureMedia quality resources or contact the team for current stock, lead time, and bulk pricing. Free worldwide shipping is available for qualified orders.