Animal Origin-Free Mycoplasma Removal Reagent
CCM-DT-0007 is a sterile-filtered liquid mycoplasma removal reagent for research cell culture workflows. Supplied as 1 set, it is animal origin-free and classified for antibiotic and antimycotic cell culture reagent use. The reagent is intended for laboratories managing mycoplasma-positive cultures within R&D, screening, and bioprocess development environments. It ships on dry ice, has a 12-month shelf life when stored as specified, and is available from CellCultureMedia with free worldwide shipping for qualifying orders.
- ✓ Free worldwide shipping
- ✓ ISO-aligned manufacturing
- ✓ Certificate of Analysis on request
- ✓ Bulk & custom volumes quoted on request
Overview
CCM-DT-0007 is a sterile-filtered, liquid mycoplasma removal reagent supplied as a 1 set configuration for cell culture reagent workflows. It is formulated as an animal origin-free antibiotic and antimycotic reagent for use when a laboratory needs to manage mycoplasma-positive cultures, supporting operational continuity while teams assess culture value, passage history, and suitability for continued use. The product belongs in detection-adjacent contamination control programs and can be specified alongside routine screening from the cell culture detection reagents portfolio.
The liquid format is designed for straightforward addition to established cell culture processes according to internal protocols and validated cell line tolerances. Because susceptibility and cellular response vary by host cell, passage number, medium system, serum level, and culture density, users should qualify dose, exposure window, and post-use monitoring in a small-scale study before applying the reagent to high-value working banks or production seed trains. The supplied size is 1 set, with list price USD 201.39.
Applications
- Research cell culture workflows where mycoplasma-positive material requires reagent-based removal before a go/no-go decision on continued culture use.
- Process development labs maintaining mammalian or other eukaryotic cell lines that need compatibility testing with an animal origin-free antimicrobial reagent.
- R&D screening environments pairing routine mycoplasma monitoring with a defined removal step for selected cultures.
- Cell bank characterization support, where small aliquots or representative cultures are evaluated before scale-up or transfer to another team.
- Academic and biopharma labs standardizing contamination response procedures across shared incubators, master stocks, and project-specific working cultures.
Storage and Handling
Store CCM-DT-0007 at -30 to -5 °C and protect the container from light. The product is shipped on dry ice to maintain frozen conditions during transit; inspect the shipment on arrival and transfer immediately to qualified frozen storage. The stated shelf life is 12 months when unopened and stored under the recommended conditions. Avoid repeated freeze-thaw cycles by aliquoting under aseptic conditions if your internal protocol permits. Use only with appropriate biosafety and cell culture handling procedures.
Quality and Documentation
This reagent is sterile-filtered and manufactured within ISO-aligned quality systems suitable for research and bioprocess development supply chains. Lot-specific documentation, including a Certificate of Analysis where available, can be requested through CellCultureMedia quality support. Procurement teams can use the CCM-DT-0007 identifier for quotation, reorder, and receiving records. For volume requirements, standing supply plans, or destination-specific shipping documentation, submit details through request a quote. Free worldwide shipping is available, with dry ice logistics applied to this frozen item.
Specifications
Storage & Handling
Storage: -30~-5 ℃, protected from light. Shelf life: 12 months. Ship cold-chain where required.
Quality & Documentation
Manufactured under ISO-aligned quality management systems. Full documentation — Certificate of Analysis (CoA), Material Safety Data Sheet (MSDS), formulation summary, and stability data — is available on request. Email [email protected] with the SKU.
About this product
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- Cell Culture ContaminationCell culture contamination falls into six categories: bacteria, yeast, filamentous fungi and moulds, mycoplasma, viruses, and cross-contamination by another cell line. Bacteria, yeast and fungi announce themselves within one to five days through turbidity, a pH shift or visible particles under the microscope, and are handled by discarding the culture and finding the technique failure that let them in. Mycoplasma, viral contamination and cell line misidentification produce no visible change at all and are found only by testing — PCR or DNA stain for mycoplasma, STR profiling for identity. The correct response to any confirmed biological contamination is to discard the affected culture, decontaminate the incubator, and restart from a clean frozen stock.
- Mycoplasma Testing in Cell CultureMycoplasma testing detects Mollicutes species that contaminate laboratory cell cultures without producing turbidity, pH change or any other visible sign, which is why a culture can carry 10⁷–10⁸ organisms per mL and still look healthy. The practical methods are PCR or qPCR (roughly 1–10 CFU/mL, results the same day), enzymatic luminescence assays (about 20 minutes, moderate sensitivity), fluorescent DNA staining read against an indicator cell line (about 10²–10⁶ CFU/mL depending on whether an enrichment step is used), and direct broth-and-agar culture, which remains the reference method but takes up to 28 days. Test cultures on arrival, after quarantine and at least monthly thereafter; a confirmed positive is normally discarded and replaced from a clean frozen stock rather than treated.
- Antibiotics and Antimycotics in Cell CultureAntibiotics in cell culture are antibacterial and antifungal agents added to growth medium to suppress microbial contamination; the standard combinations are penicillin–streptomycin at 100 U/mL and 100 µg/mL, gentamicin at 5–50 µg/mL, amphotericin B as an antimycotic at 0.25–2.5 µg/mL, and antibiotic–antimycotic 100X, which supplies all three at 100 U/mL, 100 µg/mL and 0.25 µg/mL when diluted. The professional consensus is that these should not be used routinely or prophylactically: they mask low-level contamination, select for resistant organisms, do nothing at all against mycoplasma, and can alter cell physiology and experimental response. Their defensible uses are primary tissue isolation, work with material from non-sterile sites, and short-term rescue of an irreplaceable culture — not the standard maintenance of established cell lines.
