Reverse Transcriptase ≥200 U/μL

Reverse transcriptase is used to synthesize complementary DNA from RNA templates, enabling downstream amplification, sequencing-library preparation, assay development, and process monitoring methods. CCM-DT-0003 is provided as a liquid reagent with activity specified at ≥200 U/μL and is classified as animal origin-free for workflows where material-origin documentation is important. The product is listed under cell culture reagents and is suitable for organizations standardizing molecular detection inputs across R&D, analytical development, and manufacturing-support laboratories.

The supplied configuration is 100 mL, supporting higher-throughput users and centralized reagent management. Key release specifications include sterile-filtered liquid form, pH 5.0–7.0, and osmolality 0–20 mOsmol/kg. These parameters help procurement and process teams compare lots, define incoming inspection criteria, and align internal specifications. For related molecular detection consumables and reagents, see the detection reagents category.

• First-strand cDNA synthesis from purified RNA for RT-PCR, qPCR, endpoint PCR, and sequencing-support workflows.
• Analytical method development where consistent enzyme activity, liquid format, and documented storage conditions are required.
• Bioprocess monitoring programs that require RNA-to-cDNA conversion before amplification-based readouts.
• Academic and industrial research workflows involving transcript analysis, expression profiling, and molecular assay optimization.
• Reagent standardization for multi-site laboratories needing animal origin-free classification and sterile-filtered supply.

Store CCM-DT-0003 at -30 to -5 °C and protect from light. The stated shelf life is 48 months when the product is maintained under the specified frozen storage conditions and handled using appropriate laboratory practices. The shipping condition is room temperature, so users should transfer the reagent to the required frozen storage range promptly upon receipt. Avoid repeated freeze-thaw exposure where possible by aliquoting under validated internal procedures. Before use, thaw gently, mix without vigorous foaming, and keep on cold blocks during setup according to laboratory protocol.

CellCultureMedia supplies CCM-DT-0003 for research, cell culture reagent workflows, and bioprocess support under ISO-aligned supplier quality practices. Lot-specific documentation, including a Certificate of Analysis, is available on request through the quality documentation channel. If your incoming material specification requires additional attributes such as endotoxin reporting, traceability statements, or shipping documents, include those requirements during quotation review. For volume planning, scheduled supply, or institution-specific purchasing terms, use the request quote form. Free worldwide shipping is available where permitted by destination and order requirements.