Animal-Origin-Free CHO Feed Supplement Powder

CCM-SF-0027 is a serum-free CHO feed supplement in powder form for use in animal-origin-free upstream cell culture workflows. The formulation is supplied without phenol red and without L-glutamine, reducing unwanted colorimetric interference and allowing process teams to manage glutamine addition or substitution according to internal protocols. D-glucose is included as a defined nutrient component to support controlled feeding strategies during CHO expansion and production-scale process development.

This product is specified for CHO K1, CHO-S, CHO-GS, and CHO DG44 systems operated at 37 °C with 5-10% CO2. The powder is supplied in a 10 L preparation format for laboratories that require lower shipping volume, extended inventory planning, and repeatable reconstitution procedures. For related formulations and procurement comparisons, see our serum-free media category or contact CellCultureMedia for lot availability and order planning.

• Fed-batch supplementation of CHO K1, CHO-S, CHO-GS, and CHO DG44 suspension cultures in serum-free workflows.
• Animal-origin-free process development where raw material traceability and defined formulation attributes are required.
• Scale-down model studies, shake flask screening, bench bioreactor runs, and pilot upstream process optimization.
• Culture systems where phenol red is excluded to reduce assay or optical readout interference.
• Programs requiring user-controlled L-glutamine addition, alternative glutamine strategy evaluation, or customized nutrient timing.
• Biopharmaceutical research and manufacturing support workflows focused on recombinant protein expression, cell growth profiling, and feed schedule refinement.

Store CCM-SF-0027 at 2-8 °C protected from light. The stated shelf life is 24 months when the unopened powder is stored under the recommended conditions. The product ships on blue ice to preserve controlled temperature exposure during transit. Use appropriate aseptic technique during reconstitution, and qualify the prepared supplement under site-specific procedures before routine use. Because the formulation is supplied without L-glutamine, any glutamine or substitute addition should be documented with concentration, timing, and lot information. Avoid repeated warming of the full container; where practical, prepare or aliquot according to validated internal handling instructions.

CCM-SF-0027 is supplied for research, development, and bioprocessing use with specification controls suitable for B2B purchasing review. Available release attributes include animal-origin-free classification, serum-free status, powder form, 10 L format, and endotoxin specification of ≤20 EU/mL. Manufacturing and release processes are managed under ISO-aligned quality practices, with documentation support available through CellCultureMedia. A Certificate of Analysis can be requested for lot-specific review through our quality documentation team. For volume requirements, scheduled deliveries, or purchasing approval, submit a request through request a quote. Free worldwide shipping is included to simplify landed-cost planning for international buyers.