ADCF Serum-Free Medium with Phenol Red and L-Glutamine

CCM-SF-0038 is a ready-to-use liquid medium for serum-free-media workflows requiring a chemically defined operating window and simplified procurement. It is classified as animal-origin-free, sterile-filtered, and formulated with phenol red for visual pH indication, D-glucose as a carbohydrate source, and 2 mM L-glutamine for nitrogen and energy metabolism support. The product is supplied in a 500 mL bottle for research, process development, scale-down studies, and routine cell culture use.

Key specification parameters are intended to support consistent lot qualification: pH 7.0–7.4, osmolality 270–340 mOsmol/kg, endotoxin ≤3 EU/mL, liquid form, and 12-month shelf life. The recommended culture environment is 37 °C with 5–10% CO2. Although supplied as an animal-origin-free serum-free medium, the use specification references 2% FBS where a low-serum adaptation or internal protocol requires supplementation. For adjacent formulations, see our serum-free media category.

• Mammalian cell culture development for biopharmaceutical process research and production-support studies.
• Serum-free or reduced-serum adaptation studies where phenol red and L-glutamine are preferred in the base formulation.
• Cell expansion and maintenance under 37 °C, 5–10% CO2 incubator conditions.
• Upstream workflow screening for bioreactor, shake flask, spinner, or plate-based formats, subject to cell-line qualification.
• Vaccine-process and biologics research applications requiring controlled pH, osmolality, sterility, and endotoxin specifications.
• Procurement-standardized medium supply for R&D laboratories, academic core facilities, and bioprocess engineering teams.

Store CCM-SF-0038 at 2–8 °C protected from light. Do not use after the assigned expiry date, and avoid repeated warming cycles that may affect sensitive components such as L-glutamine. Mix gently before use; do not foam or shake aggressively. The product ships on blue ice to help maintain refrigerated transit conditions. Inspect the container on receipt for closure integrity, clarity, labeling, and lot information, then return it promptly to refrigerated storage.

CellCultureMedia supplies CCM-SF-0038 under ISO-aligned quality practices with lot-level documentation available for purchasing and technical review. A Certificate of Analysis can be requested, and typical documentation may include pH, osmolality, sterility, endotoxin, shelf-life, and appearance results as applicable to the released lot. For documentation expectations, visit quality and documentation. For annual demand, multi-site supply, or price confirmation beyond the listed 500 mL price, submit a request for quote. Free worldwide shipping is available for this item.