2X Modified MEM, Phenol Red-Free

This 2X modified MEM formulation is a concentrated liquid culture medium designed for protocols that require increased nutrient density, medium blending, or user-defined dilution before use. The formulation includes L-glutamine for nitrogen and energy metabolism support, Earle’s balanced salts for ionic balance under CO2 incubation, and 9.0 g/L D-glucose for high-glucose culture conditions. It is supplied without phenol red, making it suitable for workflows where indicator dye may interfere with optical, fluorescence, absorbance, or color-sensitive readouts.

CCM-CM-0029 is supplied as a 500 mL sterile-filtered liquid and is classified animal origin-free before any user-added supplement. The source use condition is 10% FBS supplementation, with incubation at 37°C and 5–10% CO2. Release specifications include pH 7.0–7.6, osmolality 560–660 mOsmol/kg, endotoxin ≤1 EU/mL, and 12-month shelf life when stored as directed. For comparable research media formats, review the classical media category or contact procurement support through the request quote form.

• Routine culture support for keratinocytes and human melanoma-derived research cell lines when validated by the user’s laboratory protocol.
• Phenol red-free assay environments where background color or pH-indicator absorbance must be minimized.
• 2X medium blending workflows, including custom supplement addition, dilution schemes, and co-formulation with laboratory-defined components.
• High-glucose MEM-based studies requiring L-glutamine and Earle’s balanced salts under 37°C, 5–10% CO2 culture conditions.
• Scientific and basic research use in academic, biotechnology, and bioprocess development laboratories that require documented pH, osmolality, endotoxin, shelf-life, and sterility attributes.

Store CCM-CM-0029 at 2–8°C protected from light. Do not use beyond the assigned 12-month shelf life or if the container integrity is compromised. The product ships on blue ice to help maintain refrigerated conditions during transit; place the bottle at 2–8°C promptly upon receipt. Mix gently before use and avoid repeated warming cycles where possible. Because this is a 2X formulation, confirm any dilution ratio, serum addition, and supplement compatibility within the validated method used by your laboratory or process group.

CellCultureMedia supplies this medium for research and bioprocess development customers requiring consistent procurement documentation and specification transparency. Manufacturing and release workflows are ISO-aligned, and lot-specific documentation can be requested for review, including Certificate of Analysis availability, endotoxin specification, sterility status, pH range, osmolality range, and shelf-life information. See the quality documentation overview for general documentation practices. Free worldwide shipping is available for qualified orders, including refrigerated blue-ice dispatch where required for this format.