Serum-Free 293 Suspension Medium Powder

CCM-SF-0062 is specified for serum-free culture of 293F-type and related HEK293 suspension derivatives used in recombinant protein expression, viral vector process development, transfection screening, and upstream bioprocess optimization. The product is supplied as powder for preparation of 50 L finished medium, enabling centralized hydration, batch documentation, and reduced storage volume compared with liquid media. It contains phenol red for visual pH indication, D-glucose as the primary carbohydrate source, and 4 mM L-glutamine to support high metabolic demand during suspension culture.

The medium is intended for incubation at 37 °C with 5-10% CO2, matching common HEK293 suspension workflows in shake flasks, benchtop bioreactors, and scale-down engineering models. The formulation is serum-free, supporting lower raw-material variability and simpler downstream clarification than serum-containing media. The structured listing identifies an animal-origin classification; if your process requires defined-origin documentation, confirm lot-specific status before purchase. For adjacent formulations and comparison options, see the serum-free media category.

• Serum-free expansion of HEK293-derived suspension cultures, including 293F-type production lines used in transient or stable expression programs.
• Process development for recombinant proteins, antibody fragments, viral particles, and other biopharmaceutical research materials where a scalable powder medium is preferred.
• Shake-flask, spinner, wave, and stirred-tank workflows operated at 37 °C and 5-10% CO2, from R&D screening through pilot-scale parameter studies.
• Media preparation programs requiring phenol red pH indication, included D-glucose, and 4 mM L-glutamine without separate glutamine supplementation at initial make-up.
• Procurement standardization where 50 L powder packaging helps reduce cold-chain space, simplify lot tracking, and support repeated medium preparation under site SOPs.

Store unopened powder at 2-8 °C protected from light. The listed shelf life is 24 months when stored as specified and handled under appropriate laboratory controls. Shipments are packed on blue ice; inspect the container on receipt, document the lot number, and transfer to refrigerated storage promptly. During reconstitution, use validated water quality, controlled mixing, and site-approved filtration or sterilization procedures as applicable to your process. Because pH, osmolality, and sterility acceptance criteria can be lot- and preparation-dependent for powdered media, review the Certificate of Analysis and internal preparation records before releasing the medium for cell culture use.

CellCultureMedia supplies CCM-SF-0062 through ISO-aligned manufacturing and procurement controls focused on traceability, documentation, and consistent B2B fulfillment. The product specification lists endotoxin at ≤3 EU/mL and a 24-month shelf life under recommended storage conditions. Lot-specific documentation, including a Certificate of Analysis, is available on request through our quality documentation team. For volume forecasts, standing orders, or multi-site supply planning, submit requirements through request a quote. Free worldwide shipping is available for qualified orders, with temperature-controlled dispatch according to the stated blue-ice shipping condition.