Recombinant Nuclease ≥50 U/µL, 1000 µL

CCM-DT-0004 is a recombinant nuclease enzyme concentrate with an activity specification of ≥50 U/µL, supplied in a 1000 µL format for laboratories that require controlled nucleic acid degradation in cell culture, analytical, and process-development workflows. The reagent is intended for research and bioprocessing use where residual DNA or RNA reduction is part of sample preparation, lysate clarification, or upstream/downstream process characterization. As an enzyme concentrate, it is typically evaluated by users against their own buffer composition, temperature profile, incubation time, and impurity clearance criteria.

This product belongs in the detection reagents category because it supports preparation steps associated with analytical readouts, impurity monitoring, and workflow qualification. Activity is specified in units per microliter, allowing process engineers to calculate addition rates by target units, reaction volume, or biomass-equivalent input. pH and osmolality are not primary specifications for this enzyme concentrate; users should assess compatibility in the final reaction matrix. Where sterility or endotoxin limits are project-critical, confirm the release specification and available documentation during procurement.

• Reduction of nucleic acid viscosity in cell lysates, conditioned media samples, or process intermediates prior to clarification, filtration, or analytical testing.
• Sample preparation for cell culture research where DNA or RNA content can interfere with recovery, mixing, pipetting precision, or downstream assay consistency.
• Process-development studies that compare nuclease dose, incubation time, temperature, salt concentration, and hold conditions across small-scale and scale-down models.
• Bioprocess impurity-management workflows requiring defined enzyme activity input rather than undefined crude nuclease sources.
• Analytical support for R&D and QC-adjacent teams evaluating nucleic acid reduction during harvest, clarification, or intermediate processing steps.

Store CCM-DT-0004 frozen at -30 to -5 °C and protect from light. Under the stated conditions, the assigned shelf life is 24 months. Keep the container tightly closed when not in use, minimize repeated freeze-thaw exposure, and aliquot into validated working volumes if frequent access is expected. Thaw gently under controlled laboratory conditions and mix without generating foam or excessive shear. Do not use if the container integrity is compromised, if the material has been stored outside approved temperature limits, or if visible contamination is observed. Users should qualify working dilution stability within their own buffer system before routine adoption.

CellCultureMedia supplies CCM-DT-0004 for B2B research and bioprocess procurement with ISO-aligned supplier controls, batch traceability, and documentation support. A Certificate of Analysis can be requested for lot-specific review, including activity and other release information available for the supplied batch. For purchasing teams standardizing multi-site supply, our team can support quotation, pack-size planning, and documentation review through the request quote process. Additional information on quality practices is available at our quality page. Free worldwide shipping is available for eligible orders, with cold-chain packaging selected according to product storage requirements and destination conditions.